The Comparative Study Between The 85% Hepatectomy And Drug Injection For The Induction Of Rat Acute Failure Model

Objective:Compared surgery with drug induction to establish a rat acute liver failure(ALF) model,to explore a stable method of preparing a ALF model.To establish a practical and reproducible animal model of ALF,which is similar to the pathology and chemistry in human beings,in order to provide the basis for study of pathophysiologic mechanism in patients with ALF,as well as drug screening and evaluation.Methods:60 Sprague-Dawley rats were randomly divided into two groups.In operation induction group(Group A),rat ALF model was established by modified 85% hepatectomy. In the operation , 10ml 5% glucose saline solution per kilogram of body weight was injected into the left renal vein of the rat. In drug induction group (Group B),Command Carbon tetrachloride(CCl4) with tales doses arachis oil to mixed 50% solution, ALF model was established by injecting 0.9ml solution per kilogram of body weight intraperitoneally. Operative mortality and 24h survival rate after establishment of models were observed. alanine aminotransferase(ALT), Aspartate aminotransferase (AST), albumin(ALB), alkaline phosphatase (ALP), blood ammonia (NH3),total bilirubin (TBIL), prothrombin time(PT) and blood glucose (BG) were monitored.We can get light microscopic and electronic microscopic pathologic examination through liver tissue.Results:Survival rate was 0% and 30% in modified operation induction group and drug induction group 24h later, respectively. ALT,AST,ALP,NH3 and PT in the operation induction group were significantly higher than those of the drug induction group ( P < 0.05 ). TBIL,ALB and BG levels in the operation group were lower than those in the drug induction group, but the difference was not significant (P > 0.05).The 12h pathology of group A suggested not severity of the liver,But the 72h patholoty of group B suggested massive necrosis of the liver. Conclusions:The ALF model established by modified 85% hepatectomy is an ideal one. Injection of 5 % glucose saline solution through left renal vein at operation can reduce operative mortality.This model is fit for the demands of the experiment with a character of fast,comveniece,easy to contuol.The ALF model is a success.