Expression And Clinical Significance Of Gene HTERC Detected By FISH In Cervical Intraepithelial Neoplasia And Cervical Carcinoma

ObjectiveTo explore the genomic amplification and clinical significance of the human telomerase gene (hTERC) in cervical intraepithelial neoplasia and cervical carcinoma.MethodsThe cervical cytologic sample of 124 patients and out-patient from March to December 2008 , including 21 cases of normal, CINâ… 25 cases, CINâ…¡26 cases, CINâ…¢27 cases and 25 cases of cervical cancer, were collected to study the amplification of the TERC gene in cervical intraepithelial neoplasia and cervical cancer by using fluorescence in situ hybridization(FISH).ResultsThe successful hybridization rate by using FISH is 80%; the amplification of the TERC exist in normal, CINâ… , CINâ…¡, CINâ…¢, cervical cancer, and the positive expression rates are 4.76%, 12%, 76.92%, 85.19%, 100%, respectively. The results show that there is no significant amplification of TERC in normal and CINâ… (p>0.05),but with a higher amplification in CINâ…¡and above lesions(p<0.05). In CINâ…¡and above lesions, the sensitivity rate of TERC amplification is 87.18% , with a specificity rate of 91.30%, a positive predictive value of 94.44% and a negative predictive value of 80.77%. The sensitivity rate between TCT and TERC amplification shows significant difference, while the specificity rate between them show no significant difference. Besides, the sensitivity rate between the high-risk HPV infection and TERC amplification shows no significant difference, while the specificity rate between them show significant difference.From normal to cervical cancer, the increasing gene copy numbers of TERC is in company with the predominantly integration of high-risk HPV , which shows obvious relevance (r=0.955,P<0.05). ConclusionsThe amplification of TERC may play an important role in the occurrence and development of cervical cancer., as a significantly amplification of TERC exist in CINâ…¡and above lesions, while few amplification was found in normal and CIN1.In the development of precancerous lesions, TERC gene amplification shows a closely relation with high-risk HPV infection. By detecting TERC gene amplification to screen for cervical cancer and precancerous lesions, FISH technology shows a higher sensitivity and specificity.