The Inhibition Effect Of ShRNA On VEGF-C In Breast Cancer Cells

Objective: To investigate the effect of the RNA interfering on VEGF-C in MDA-MB-231 cells.Methods: The three small interfering RNA(siRNAa,siRNAb,siRNAc) were prepared. The most efficient gene was screened and short hairpin RNA(shRNA) was designed, the recombinant plasmid pGenesil-1/VEGF-C was constructed, and transfected into MDA-MB-231 cells by Lipofectamin 2000. RT-PCR, Western blot and immunocytochemical methods were performed to detect the expression of VEGF-C.Results: RT-PCR results showed that siRNAa,siRNAb,siRNAc could inhibit the growth of MDA-MB-231 cells, among of which, siRNAa was the most significant, the inhibition rate was 72.1%. The recombinant plasmid pGenesil-1/VEGF-C was successfully constructed using shRNA and pGenesil-1. VEGF-C expression was significantly inhibited by RT-PCR,immunocytochemical methods and Western blot(P<0.05).Conclusions: shRNA RNAi technology could silence the expression of VEGF-C in MDA-MB-231 cells, as suggested that the technology may be one of effective methods for inhibiting lymphangiogenesis in breast cancer.