Proteomic Analyse Of Organ-Cultured Artery Under Different Shaer Stress

Vascular remodeling is the common pathological foundation of cardiovascular diseases including atherosclerosis and hypertension, during which low shear stress plays as a crucial factor. However the molecular mechanisms of this process are not fully clarified. The related proteins or biomarkers are identified by proteomics analysis during the shear-stress-induced vascular remodeling, which would deep our understanding on the mechanism of vascular remodeling and provide practical guidance for the prevention and diagnosis of cardiovascular diseases.In the present study, aortas of rats were exposure to normal shear stress (15 dyn/cm2) and low shear stress (5 dyn/cm2) respectively for 24 hours in a vessel culture system in vitro. Proteomics techniques were conducted to investigate and indentify the differentiated expressed proteins of the cultured vessels. Then,real time PCR and western blot were used to verify the expression of Rho-GDIα, a protein indentified previously in proteomics, on the vascular tissue. And parallel-plate coculture flow chamber system and western blot were applied to assess Rho-GDIαexpression in vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) respectively under normal and low shear stress.The results showed that 78 differentiated expressed proteins were found after 2-DE gels analysis, 43 proteins'IDs were identified through MALDI-TOF-MS, and most of them were functionally involved in cell cytoskeleton, signaling, metabolism and extracellular matrix;compared to normal shear stress, both mRNA and protein expression of Rho-GDIαwere relative lower under low shear stress in cultured aorta; Rho-GDIαwere also suppressed both in VSMCs and ECs under low shear stress, which was consistent with the previous finding in proteomics and verification on the tissue level.These results indicate that proteomics may provide an insightful angle to demonstrate the mechanism of shear-stress-induced vascular remodeling, and Rho-GDIα, one of our indentified proteins, may participate in the process of low-shear-stress-induced vascular remodeling.