| Background: Systemic inflammatory response syndrome (SIRS) is the prophase of clinical manifestation for a lot of disease which will chang into multiple organ dysfunction syndrome (MODS) or MSOF. It finally could cause these diseases to get worse. Acute pancreatitis is one of the important disease causing SIRS. Polymorphonuclear neutrophils (PMN) is the main effector cell in the development of inflammatory, it plays a key role in SIRS, for which influence occurrence, development and turnover. PMN will die by means of apoptosis under the normal condition, it is identified phagocytized and cleaned by macrophage phagocytosis. If apoptotic PMN will not be phagocytized and cleaned by macrophage promptly, the toxic substance released by disintegrated apoptotic PMN causes tissue trauma and through infernal circle makes SIRS to aggravate. Therefore, it is particularly important to clear away PMN apoptosis timely and moderate. Membrane bound form CD14 (mCD14) is one of the main receptor of MΦrecognition and phagocytosis of apoptotic neutrophil.Objective: In this study, the aim is to study the exosomatic function of applied rheum emodin to SIRS in severe acute pancreatitis in SD rat peritoneal macrophage mCD14 through making the SIRS SD rat model. To investigate the meaning of which peritoneal macrophages distinguish and swallow apoptotic PMN of SIRS in severe acute pancreatitis. And provide a theoretical basis for clinical drug therapy.Methods: The SIRS model of SD rat was established by retrograde injection of 1.5% concentration of sodium deoxycholate into common biliopancreatic duct. SIRS model of severe acute pancreatitis. Preparation of animal model:20 SD rats were randomly divided into 2 groups, that is, sham operation group,model group of SIRS in SAP, 10 SD rats each group. Experiment of intervention in vitro:Macrophage, PMΦ dosing stage: Macrophages which were taken out from sham-operated group were divided into a separate group .PMΦwhich took out from SIRS in SAP model group was divided into 3 groups. That is, emodin intervention group,Dexamethasone intervention group,Drug blank control group. Emodin intervention group (Final intervention consistency: 5ug/ml, final ethanol consistency <0.1%); Dex intervention group (Final intervention consistency: 0.1umol/ml), each intervention group was dosed emodin after macrophages adherent (MΦculture 24h).Selected samples made pathological examination and object detection after making model 24h.Each group were observed pathological changes of pancreatic specimen and made histological score. The different levels of mCD14 expression in different groups were analyzed by semiquatitative reverse transcriptase polymerase chain reaction.Results:1.Pathological alteration of pancreas with microscope:compared with sham operated group, the pathological alteration of pancreas of SIRS model group is significantly changed(edema : 3.50±0.21vs0.40±0.07,inflammation : 2.70±0.16vs0,hemorrhage:2.30±0.19vs0,necrosis:2.80±0.25vs0,P﹤0.01);2. Comparison of pMΦmCD14 expression of SD rats in each group(1) Compared with the sham-operated group:the expression of pMΦmCD14 of SIRS model group declines obviously(28.55±2.53vs14.76±2.84,P﹤0.01);(2) Compared with the SIRS model group: the expression of pMΦmCD14 of emodin intervention group is increased obviously(25.60±2.79vs14.76±2.84,P﹤0.01); the expression of pMΦmCD14 of dexamethasone intervention group is increased obviously(20.87±1.99vs14.76±2.84,P﹤0.01)。(3) Compared with the dexamethasone intervention group: the expression of pMΦmCD14 of emodin intervention group is increased obviously(25.60±2.79 vs 20.87±1.99,P﹤0.01).Conclusions: Compared with sham operated group, the pathological alteration of pancreas in SIRS model group was changed at extent severely.The expression levels of mCD14 also decreased significantly.When SIRS in severe acute pancreatitis,the expression of pMΦmCD14 of rats declines obviously. Each intervention group improve varying degrees, especially in emodin intervention group.
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