The Effect Of Chitosan On The Endothelial Regeneratioin And Neointimal Proliferation After Balloon Angioplasty Of Arterial In Rat

Objective :To investigate the effect of chitosan on the endothelial regeneratioin and neointimal proliferation after balloon angioplasty of right carotid arterial in rat。Methods: Endothelial denudation of arterial in rat were performed with 2F balloon catheter. 50 SD rats were randomly divided into the control group and the chitosan group. Arterial were harvested on 0 day;7 days;14 days;28 days and 35 days after the injury. The reendothelialization and the neointimal hyperplasia were observed cross-sections stained with hematoxylin-eosin from the injured segments processed for histological and morphological study by computer-assisted picture analysis system.Result: chitosan enhanced the reendothelialization of the injured arterial determined VEGF staining. The absolute reendothelialization was observed in 14 days in chitosan group but in 28 days in the control group. Intimal hyperplasia and lumen stenosis can be seen on the 7 days after injury. At the 28th day the intimal thickening reached at a peak. In the chitosan group the intimal thickening reached at a peak at the 14th day. There were significant difference of neointimal in the chitosan group and the control group on the 14 th day;28 th day and 35 th day .Conclusions: chitosan is effective on intimal hyperplasia and lumen stenosis after arterial injury by inhibiting neointimal proliferation which is related to the reendothelialiazation.