Experimental Study Of Effects Of The Inorganic Induced Elements Bone Scaffold Material On Growth And Differentiation Of Osteoblast From Periosteum

Objective Osteoblat is ideal seed cell in bone tissue engineering.Culturing osteoblast in vitro has comprehensive and applied value.Osteoblast is cultured from periosteum and divided into 2 groups.We discuss the effects of the inorganic induced elements scaffold material on growth and differentiation of osteoblast by comparing bone formation of the 2 groups.Methods Osteoblast is separated and cultured with enzyme digestion from periosteum and then divided into 2 guoups randomly.The inorganic induced elements scaffold material is added into A group(experimental group),and the other group(B group) is control one.Then we compare the morphology and bone formation character between the 2 groups.Results Cells in both groups appear morphous and growth character of osteoblast. In A group there are 94.9±0.8%cells can be colored by alkaline phosphatase,however in B group only 91.2±0.1%,ALP in experimental group clear supernatant liquid is 2.91±0.74u/ml,the other is 2.01±0.22ng/ml.In experimental group it is about 14 days when calcium nodus appeared after cell fusion.In control group it is 17 days. Osteocalcin in experimental group clear supernatant liquid is 3.217±0.254 ng/ml.the other is 2.865±0.235ng/ml.Conclusion Periosteum has good bone formation ability.The inorganic induced elements scaffold material can well facilitate growth and differentiation of osteoblast from periosteum.