Association Of X Gene C1485T Mutation With Hepatocellular Carcinoma In Chronic Hepatitis B Virus Infection

Background/Aims:Hepatocellular carcinoma(HCC) is one of the common and malignant tumors in China.It have a high morbidity,and the prognosis is extremely poor.Hepatitis B virus(HBV) and Hepatitis C virus(HCY) infection,environmental factors and the genetic factors are associated with HCC.Especially,HBV infection is the major factor.Currently,HBV has been classified into eight genotypes with distinct geographic distributions.HBV genotypes B and C are prevalent in China,and previous studies have suggested that genotype C is associated with more active hepatitis,more advanced liver disease,and higher prevalence of HCC than genotype B.In addition,the HBV X gene,which encodes the pleiotropic transactivater HBx,is also associated with the development of HCC.Epidemiological investigation showed that the mutations and the deletions of HBx which enhance its transactivation function have been found to increase the risk of HCC development.It has been reported that the mutation at C1485T of HBV X gene in the patients of HCC have high morbidity.However,the association of HBx gene C1485T mutation with HCC is still controversy.In this study,we investigate the prevalence of C1485T in chronic HBV infection and risk factors associated with the development of HCC.Methods:1.90 cases of HBV related HCC and 90 cases of chronic hepatitis B and hepatitis B liver cirrhosis were investigated.Their serum and clinical data were collected.PCR was performed for the amplification of HBV DNA.The mutation of C1485T was tested by PCR-RFLP assay.And then the PCR amplification products were purified using PCR purification kit.At last, we used the gene sequencing technology to verify the results.2.Data were analyzed by SPSS13.0 software.The numerical variables were analyzed by T test and Mann-Whitney test.And the categorical variables were analyzed by Chi-square test.Multiple logistic regression were used to analyze the risk factors associated with the development of HCC(p＜0.05).Results:The mutation of C1485T in HBx gene was found in 24/50(48%) patients with HCC,while in 13/90(14.4%) patients with chronic hepatitis B(CHB) and liver cirrhosis(LC).The mutation rate between the two groups is statistically difference(p＜0.0001).Multiple logistic regression analysis showed the prevalence of the C1485T change was significantly associated with HCC.(P=0.037,odds ratio:3.146).Conclusion:The C1485T change in genotype C is an independent risk factor for the development of HCC and may serve as a useful molecular marker for predicting the clinical outcomes in patients with chronic HBV infection.