| Objective: To observe the effect on TC,TG,HDL,LDL,ApoA,ApoB,thromboxane B2,6-keto-PGF1α and aortic endothelial cells of HLP combined AS rabbits treated by herbal-cake-separated moxibustion and to analyze the anti-hyperlipemia rationale of the therapy.Method: While fed with high fat food everyday and injected with BSA, HLP combined AS rabbits were treated with different way of direct and herbal-cake-separated moxibustion on the same acupuncture points.Results: (1) Under transmission electron microscope, observation of aortic endothelial cells shows: endothelial cells in direct-moxibustion group and herbal-cake-separated-moxibustion group are intacter, most of them are normal in forms, internal elastic membrane is continuous, thickness is even, the cells of muscles of medial membrane are relatively normal and the structure of aortic endothelial cells of those groups are similar to those of blank group. But the structure of aortic endothelial cells in model group is different , endothelial cells disappeared, in cytoplasm the sedimentation of a great quantity of lipide can be seen, internal elastic membrane is interrupted, thickness is uneven, there is focal dissolution, the cells of smooth muscle in the medial membrane has sedimentation of lipide, and has frothy change.(2) Compared with model group , the TC and TG content in herbal-cake-separated-moxibustion group and direct-moxibustion group is obviously lower (P<0.01 ). Compared with direct-moxibustion group, TC content in herbal-cake-separated-moxibustion group is obviously lower(P<0.01).(3) Compared with model group , the HDL content in herbal-cake-separated-moxibustion group,direct-moxibustion group and blank group is obviously higher (P<0.01, P<0.05; P<0.05), the LDL content is obviously lower (P<0.01; P<0.01; P<0.01), the ratio of HDL/LDL and the ratio of HDL/TC is obviously higher (P<0.01; P<0.05; P<0.05).(4) Compared with model group , the ApoA content in herbal-cake-separated-moxibustion group,direct-moxibustion group and blank group is obviously higher (P<0.01, P<0.01; P<0.05), ApoB content is obviously lower (P<0.01; P<0.01; P<0.01), ratio of ApoA/ApoB is obviously higher (P<0.01; P<0.01; P<0.01).(5) Compared with model group , the 6-keto-PGF1α content in herbal-cake-separated-moxibustion group,direct-moxibustion group and blank group is obviously higher (P<0.05; P<0.05; P<0.05),(6) Compared with model group , the TXB2 content in herbal-cake-separated-moxibustion group,direct-moxibustion group and blank group is obviously lower (P<0.05; P<0.05; P<0.05).(7) Compared with model group, the ratio of TXB2/6-keto-PGF1α in herbal-cake-separated-moxibustion group, direct-moxibustion group and blank group is obviously lower (P<0.01; P<0.01; P<0.01).Conclusion:(1) Content of TC,TG,LDL and ApoB of HLP combined AS rabbit is decreased after treated with direct-moxibustion or herbal-cake-separated -moxibustion , while HDL content is increased. It points out direct -moxibustion and herbal-cake-separated-moxibustion has favorable regulated effect to metabolism of lipide and lipoprotein.(2) In decreasing effect on TC of HLP combined AS rabbits , herbal-cake-separated-moxibustion is better than direct-moxibustion,(3) Herbal-cake-separated-moxibustion has regulating effect to the content of 6-keto-PGF1α,TXB2 and ratio of T/P of hyperlipemia rabbits,,(4) Herbal-cake-separated moxibustion has protective effect to the aortic endothelial cells of hyperlipemia rabbits. |
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