Separation Of Sync Of Flavonoids, Alkaloids And Polysaccharides &. Researches Of Function Of Alkaloids In Lotus Leaves

In recent years,lotus leaves as a new resource of plants were researched hotly. There were a lot of active substances beneficial to body health,such as flavonoids, alkaloids and polysaccharides.The separation of sync of the three active ingredients in lotus leaves and the interaction between alkaloids extractive and DNA,BSA were investigated.The process method of separation is 1.the process parameters of flavonoids extracted from the dry powder of lotus leaves is pH=13.0,ethanol concentration=50%, solid-liquid ratio=1:30,T=75℃,t=2h,extract once;the process parameters of alkaloids extracted from the residue is pH=2.0,ethanol concentration=90%,solidliquid ratio=1:25,T=85℃,t=2h,extract twice;the process parameters of polysaccharides extracted from the residue by water is pH=7.0,solid-liquid ratio=1:30,T=75℃,t=2h,extract once.Brown powder was gotten after purification of flavonoids extraction by AB-8 macroporous resin,the purity of 84.43%,yield of 90%.Yellow-green powder was gotten after purification of alkaloids extractive by D101 macroporous resin,the purity of 82.76%,yield of 90.21%.White powder with slightly yellow was gotten after purification of polysaccharides extractive by removal of protein and decolorization,the purity of 85.74%,yield of 85.81%.The study provided a scientific basis for the lotus leaf as the development and utilization of health food products.The research to make full use of the natural resources of plants -lotus leaf is of important scientific significance.An accurate determination of alkaloids in lotus leaves was set up by using HPLC in the neutral conditions.The molecular weights of five main components of alkaloids in lotus leaves were determined by mass spectrometry as M_A265,M_B311, M_C281,M_D295 and M_E311,they are speculated as Anonaine,Pronuciferine, N-Nornuci- ferine,Nuciferine and Armepavine respectively.In the selected two-phase solvent system,an successfully HSCCC separation and purification of alkaloids in lotus leaves was investigated,the optimal conditions is T=31℃,spiral speed=850 rpm,flow rate=1.8 mL/min,by HPLC detection,the purity of five components separated were determined:Anonaine,99.6%; Pronuciferine,91.2%;N-Nornu- ciferine,98.5%;Nucferine,99.3%;rmepavine, 96.1%.The interaction between nuciferine,alkaloids extractive and DNA,BSA were investigated.It is found that they can react with DNA under physiological condition (pH=7.4).The interaction mode was of a mixed one that the inlay and groove binding were the two major modes.The quenching model belonged to static fluorescence quenching.The binding constant for nuciferine,alkaloids extractive with DNA was 1.396×10~6 L/moL and 3.765×10~6 L/mol.And it is found that they can react with BSA under physiological condition(pH=7.4) too.The quenching model belonged to static fluorescence quenching.The binding constant for nuciferine,alkaloids extractive with BSA was 5.194×10~4L/g and 1.127×10~5L/g.A theoretical basis for an in-depth study of alkaloids of the drug development and drug screening was provided.