The Age-related Changes Of Asymmetric Dimethylarginine,DNA Methylation And The Relationship Between Them And Kidney Aging

ObjectTo observe the age-related changes of plasma asymmetric dimethylarginine (ADMA) and global DNA methylation in healthy people. To evaluate the relationship between ADMA and global DNA methylation. Make glomerular filtration rate (GFR) as a early sign of renal age-related changes, and to evaluate the relationship between ADMA, global DNA methylation and GFR, in order to find whether ADMA or global DNA methylation participate the progress of renal age-related changes.MethodsHealthy participants (n=80) screening with history-taking, physical examination and laboratory tests were divided into four age groups : A(32-41years), B(42-51years), C(52-61years) and D(≥62years), the proportion of male and female was 1:1. GFR was estimated by the modification of diet in renal disease (MDRD). DNA methylation was measured through High Performance Liquid Chromatography. ADMA was measured though Liquid chromatography - mass spectrometry.Results1. ADMA increased and global DNA methylation decreased with age. Compared to the other three groups, decrease of global DNA methylation was significant statistically in D group (P < 0.05). The increase ADMA in group D was significant statistically compared to A and B groups (P < 0.05).2. The increase of ADMA was not correlated with the decrease of global DNA methylation (P>0.05).3. Increase of ADMA and decrease of global DNA methylation were all not correlated with GFR (P>0.05).Conclusions1. ADMA increased and global DNA methylation decreased with age, which indicated that they participated the aging process.2. Increase of ADMA had no relationship with decrease of global DNA methylation, which indicated that global DNA methylation had no effect to ADMA increased.3. Increase of ADMA and decrease of DNA methylation had no relationship with GFR, which indicated that ADMA or global DNA were not the sensitive indicators of the kidney aging.