Study On The Activation Effects Of Angiotensin Ⅱ On Periodontal Ligament Cells

ObjectiveThe identification and the development of potential growth factors for the cells of the periodontal ligament is an important and novel stratagem for the regeneration of tissue destroyed by periodontitis and periapical inflammation. The problem is that growth factors are difficult to synthesize, very immunogenic and expensive. The purpose of our study was to determine whether angiotensin II, a naturally occurring octo-peptide with growth factor like properties in cardiac muscle, could also stimulate cultured human periodontal ligament cells.Methods(1) Periodontal ligament cells were cultured and identified in vitro by explant method.(2)Cell counting and MTT assay were used to study the effects of angiotensin II on the proliferation and activity of cultured cells.(3)A wounding periodontal ligament cell model in vitro was established. The distance of cells migration was observed and measured by cultured with angiotensin II and losartan and analysed by photoshop software. (4)Collagen I synthesis of periodontal ligament cells was determined by immunofluorescence technique. The semi-quantitative analysis was made to collagen by image pattern analysisor.Results(1)Angiotensin II can promote the proliferation and activation of periodontal ligament cells through AT1 receptor. The effect can be blocked by losartan.(2)The cells cultured with angiotensin II migrated faster than those in common cultured or cultured with both losartan and angiotensin II.(3) Angiotensin II can promote the collagen I synthesis. Losartan can block this effect.Conclusions(1) Periodontal ligament cell is the target cell of angiotensin II.(2)Angiotensin II can promote the proliferation, migration and collagen I synthesis of periodontal ligament cells. The effects can be blocked by losartan.