| Objective:To investigate the effect of sevoflurane and propofol on lung ischemia-reperfusion injury in rabbit in vivo.Methods:Forty healthy rabbits of either sex weighing 1.5-2.5kg were randomly divided into four groups of ten each:Compared(group A):Chest was opened and left main bronchus and pulmonary artery and vein were isolated but not clamped.The lungs were ventilated for 180 min;Ischemia-reperfusion(group B):left hilum was isolated and clamped for 60min,after declamping the lungs were ventilated for 120 min;Sevoflurane+ ischemia-reperfusion(group C):the lungs were ventilated with 1 MAC sevoflurane for 180 min,during this period,left hilum was isolated and clamped for 60min,then declamping for 120min.Propfol+ ischemia-reperfusion(group D):Propofol was lasting infused into intravenous,during this period,left hilum was isolated and clamped for 60min,then declamping for 120min.Results:1.Changes of SOD active unit and MDA content:①The SOD active unit of left lung was signifigantly lower in group B than those in group A,group C and group D(P＜0.05).Compared with group C and group D, the SOD active unit of left lung was signifigantly higher in group A(P＜0.05).There was no significant distinct between group C and group D(P＞0.05).②The MDA content of left lung was signifigantly higher in group B than those in group A,group C and group D(P＜0.05).Compared with group C and group D,the MDA content of left lung was signifigantly lower in group A(P＜0.05).There was no significant distinct between group C and group D(P＞0.05).2.Changes of W/D ratio and LPI:①The W/D ratio of left lung was signifigantly higher in group B than those in group A,group C and group D(P＜0.05).Compared with group C and group D,the W/D ratio of left lung was signifigantly lower in group A(P＜0.05).There was no significant distinct between group C and group D(P＞0.05).②LPI of left lung was signifigantly higher in group B than those in group A,group C and group D(P＜0.05).Compared with group C and group D,LPI of left lung was signifigantly lower in group A(P＜0.05).There was no significant distinct between group C and group D(P＞0.05).3.The category and counting of WBC in BALF:The counting of WBC and PMN cells of left lung was signifigantly higher in group B than those in group A,group C and group D(P＜0.05).Compared with group C and group D,the counting of WBC and PMN cells of left lung was signifigantly lower in group A(P＜0.05).There was no significant distinct between group C and group D(P＞0.05).Conclusions:1.The lungs structure was severely destroyed when the lungs suffered LIRI. 2.Sevoflurane and propofol could protect the lungs against LIRI in rabbit in vivo,and there was no significant distinct between them.3.Neither Sevoflurane could completely protect the lungs against LIRI in rabbit in vivo nor propofol. |
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