Iron Metabolism Disorders And The Mechanisms Of Promoting The Liver Cirrhosis In Cirrhosis Portal Hypertension Hypersplenism Patients

Objective: Through detecting the iron content of liver and spleen, liver tissue transferrin receptor level in protein and gene expression and blood in the expression of tumor necrosis factor of Portal hypertension in cirrhotic hypersplenism, Preliminary study to disorder of iron metabolism and promote the mechanism of liver cirrhosis in varying degrees of hypersplenism of portal hypertension in cirrhotic patients with hypersplenismMethods: Experimental grouping: Healthy control group, mild hypersplenism group, moderate hypersplenism and severe hypersplenism Group;before and after operation in each group self-control. Using RT-PCR and immunohistochemical methods detecting of transferrin receptor level in gene expression and protein levels; Atomic Absorption Spectrophotometer method discover spleen, liver tissue iron content; ELISA assay of serum liver cirrhosis-related cytokines tumor necrosis factor (TNF-α) expression; Application of SPSS 13.0 statistical software for analyzing the medical gene and protein expression differences and correlation.Results: Liver and spleen iron content group organizations to compare three groups of liver cirrhosis group than the normal group, The results were significantly different(P<0.05). Hypersplenism with mild to moderate iron content in the spleen group there was no significant difference (P>0.05), May be related to the number of cases is too small, The rest of the iron content of the comparison group were significantly different (P<0.05); the larger of the degree of hypersplenism the larger of the iron content in the liver organizations. The increased iron content in the spleen and liver is significant correlation (correlation coefficient r=0.741, P<0.01); RT-PCR results showed that the expression level of TfRmRNA groups are statistically significant results (P<0.05), Hypersplenism TfRmRNA expression groups were higher than the normal control group, Three groups with hypersplenism hypersplenism between the degree of understanding, TfRmRNA followed by increased expression (P<0.05); Liver tissue immunohistochemical staining results of TfR protein expression than the normal control group in three groups Hypersplenism, No obvious change in surface density(P<0.05), Significantly reduced the average gray level, there was a significant difference(P<0.05), Integral optical density increased, there was a significant difference (P<0.05); the results of tumor necrosis factor (TNF-α) in blood samples content are differences between the groups were significant (P<0.05), three sets of samples in cirrhotic portal hypertension were higher than normal group, With increasing the degree of hypersplenism, Tumor necrosis factor (TNF-α) in order to increase absorption (P<0.05). Compared preoperative and postoperative group were statistically significant in hypersplenism(P<0.05). There is an obvious positive correlation in that TNF-αexpression in peripheral blood increased with increasing the iron content of liver and spleen organizations(P<0.01).Conclusions: Hyperfunction of the spleen lead to liver damage and liver iron overload in cirrhosis portal hypertension hypersplenism patients; Iron overload caused by the expression of TNF-αincreased, causing liver fibrosis and sclerosis; The results of this study support the early removal of spleen of moderate and severe hypersplenism.