Experimental Study Of The Inhibition Effection Of Cisplatin-chitosan Drug Delivery Film For Nude Mice Peritoneal Sub-clinical Transfered By Gastric Cancer

Objective: Investigation the inhibitory action of chitosan-DDP drug delivery film to gastric cancer peritoneal sub-clinical transfer by intraperitoneal chemotherapy。Methods: Blend DDP and chitosan in wight 1:3, Chotisan dissolves in 1.5% acetic acid to compound into 1.5% chotisan solutions,cross-link with glutaraldehyde, modified with gelatin and glycerol ,and then drooling on Polytetrafluoro ethylene board, adding cisplatin and vacuum drying. The same mehthod prepared drug-free chitosan as control group. Take three pieces cisplatin-chotisan drug delivery films immerses in HCL dissolution completely test cisplatin contents of the films by ICP-AES and one piece in 5ml RPMI 1640 medium, ICP-AES test the 1st ,2nd ,3rd ,4th ,5th ,7th days respectively of the cisplatin contents extracted from the cisplatin-chotisan drug delivery film . The SGC-7901 gastric cancer cell were cultured in RPMI1640(add 10%FBS),5%CO2,humidity 95%,37℃Incubator,at the time of Logarithmic phase, Trypsin digestion and then centrifugalize, Adjust the concentration of cells to 1×107, All 32 nude mice were intraperitoneal injected SGC-7901 gastric cancer cells 0.2ml1X107 and then divided into four groups randomly: chitosan-DDP Film group,chitosan Film group,cisplatin group and NS group. feed in SPF condition two weeks and then assess the effects of IPC by chitosan-DDP Film. to observe body weight,the number of cancer nodes,two weeks survival rate, mensuration the platinum concentration of the Omentum.Results: The film drug encapsulation rate and drug loading rate were 42.37±3.9% and 6.8±0.5%, releasing test in vitro of chitosan-DDP Film showed that the platinum concentration was 154.20μg/ml in the 1st day, then the platinum releasing stably. platinum concentration of extracts still reaches to 4.13μg/ml in the 7th day: the body weight IPC by chitosan-DDP Film were 20.60±1.98g, the number of cancer nodes were 3.50±1.51,,two weeks survival rate were 100%, compare with other groups showed statistical significance(p<0.05), and the platinum concentration of the Omentum were 1247.23±179.46 ng/g, higher than the group of simple IPCT by DDP only (P<0.05).Conclusions: The chotisan-DDP drug delivery films show well Drug encapsulation rate and drug loading rate in vivo and vitro.IPC by the chotisan-DDP drug delivery films can improve the curative effect obviously, show perfect effect to inhibition of gastric cancer peritoneal sub-clinical transfer.