Study On The Level Of Interleukin-23 In Cerebrospinal Fluid Of Patients With Tuberculous Meningitis

Objective:Interleukin-23(IL-23) is an important cytokine which adjusts cellular immunity. Mycobacterium can make macrophage, which clear the mycobacterium, secrete high level of IL-23 to promote cellular immunity. But there is no report about the act of IL-23 in tuberculous meningitis(TBM). We examined the IL-23 levels in the cerebrospinal fluid (CSF) from the patients with TBM and non-TBM (which include cryptococcus meningitis and viral meningitis), and explored its clinical values and possible roles in the pathogenesis of TBM.Methods:The IL-23 levels in CSF were measured by a double-antibody sandwich ABC-enzyme-linked immunosorbent assay (ABC-ELISA) in 29 TBM patients and 25 non-TBM patients. The IL-23 levels in CSF from TBM and non-TBM patients were contrasted to find whether there is diversity between the two groups. Parameters of biochemistry function within CSF including Protein, Glucose and Chloride were detected by automatic biochemistry analyzer. Meanwhile, White Blood Cell (WBC) were measured by Urinary sediment autoanalyzer. Then, we analyzed the association between IL-12 and clinical parameters including Protein, Glucose, Chloride and WBC.Results:1. The IL-23 levels in CSF from TBM patients is 100.97±120.27pg/ml, significantly higher than non-TBM patients (P<0.05).2. There was no obvious correlation with IL-23 Level and clinical parameters (P>0.05).Conclusions:1. IL-23 may attend the pathological progress of TBM.2. The measurement of IL-23 levels might provide clinical clues and theoretical basis.