Effects Of Antisense Oligonucleotide Targeting Hsa-miR-518b On The Growth Of Gastric Cancer Cell

Objective To investigate the effects of antisense oligonucleotide (ASODN) targeting hsa-miR-518b on the growth and cycle of gastric cancer cell, and the mechanism in gastric cancer.Methods Specific phosphorothioate antisense oligodeoxynucleotides targeting hsa-miR-518b were synthesized and then transfected into the gastric cancer cell(SGC7901). We investigated the change of the hsa-miR-518b expression in SGC7901 after the transfection by using real time RT-PCR. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell growth of SGC 7901 and Flow cytometry (FCM) was used to analyze the cell cycle of SGC7901.Results After transfection, the expression of hsa-miR-518b was decrased(p<0.01), the growth of SGC7901 was suppressed(p<0.01) and the cell cycle arrested in G1(p<0.01).Conclusions hsa-miR-518b(ASODN) could suppress the growth of SGC 7901 by arresting the cell cycle in G1 phase. Hsa-miR-518b could promote the growth of gastric cancer cell by regulating Gl/S transition.