The Expression Of Protein VEGF And TGF-β₁ In The Process Of Cicatrization Of Incised Wounds On Rat Skin

Objective:To investigate whether the expression of protein VEGF and TGF-β1 can be used as an index to estimate injury age in the field of forensic medicine by approaching the temporal relation of VEGF and TGF-β1 expression with injury in the healing process of the incised wounds on rat skin.Methods :Using rats to establish experimental models by making incisions on the rats skin, dividing them randomly into three groups: the experimental group, the normal group and the postmortem injured group,followed by observing the expression of VEGF and TGF-β1 with Immunohistochemical techniques in their process of cicatrization.Results:In experimental group,the expression of VEGF and TGF-β1 was higher than that in the normal control and postmortem injury group (P<0.05). The expression of VEGF mainly exists in the neutrophilic granulocyte, macrophages and fibroblasts 3-24h after injury, and its positive level increase in direct proportion to the wound age. Two days after the injury, the presence of VEGF is observed in the macrophages, fibroblasts, and the vascular endothelial cell of neoformative granulation tissue with strong positive expression, reaching a summit on the 4thday after the injury. The positive cells in both groups will then gradually decrease, with the protein expression turning weakly positive. Their difference with the normal control group is significance(P<0.05). The changing rule of TGF-β1 expression in experimental group is similar to that of the VEGF expression.Conclusion:The expression of VEGF and TGF-β1 during the cicatrization process follows temporal rules, thus can be provided as an new index to judge the Injury age in the field of forensic medicine.