Effects Of Total Glucosides Of Paeony On The Expression Of IFN-γ,TNF-α And IL-10 In The Ratmodel For Chronic Abacterial Prostatitis

Objectice To investigate the role of IFN-γ,TNF-αand IL-10 in the rat model for chronic abacterial prostatitis( CAP) and study the effects of total glucosides of paeony ( TGP) on the IFN-γ,TNF-αand IL-10 in the rat model and the immune mechanism and immunotherapy of CAP which provide a new pathway for the clinical treatment of CAP.Methods 40 Aged Sprague Dawley rats were randomly divided into four groups : the control group(10 rats), the rat model for CAP group(10 rats), the rat model for CAP treated with low dose TGP group(10 rats) and the rat model for CAP treated with high dose TGP group(10 rats). The changes of prostate gland inflammation was investigated by HE stain .The expression of IFN-γand TNF-αin rat prostatic tissue was detected by immunohistochemistry(sp) .T he expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay (ELISA)Results (1) The changes in histopathology in the animal model were similar to those in clinical CAP and the inflammation of treatment group obvious change for the better. (2) The IFN-γ,TNF-αand IL-10 in the rat model for CAP group were higher than the control group (P<0.01). (3) The IFN-γ,TNF-αand IL-10 expression reduces along with the TGP dosage increases in the rat model for CAP. (4) Between each group the IFN/IL-10 and TNF/IL-10 have statistics significance (P< 0.01).Conclusion The IFN-γ,TNF-αand IL-10 may play an important role in the development of the CAP and CAP may be caused by abnormal autoimmunity. TGP has therapeutic effects on CAP rats , and its mechanisms may be associated with its ability to ameliorate the abnormal immunological functions and cytokine secretion in CAP rats.