| Introudution: Asthma is a chronic airway inflammatory diease, whose mechanism is very complex involved many kinds of inflammatory cells, medium and cytokines. Adhesion moleule develop positive effectiveness in the early stage of inflammation of asthma. Glucocorticoids is the most effective drug in asthma threapy, but it may produce adverse reaction or dependence to some people. Oxymatrine is a kind of traditional Chinese medicine with anti-inflammation effectiveness, the mechanism of anti-asthmaic may concerned with decrease the expression of adhesion moleule.Objective: To study the expression changes of intercellular adhesion molecule(ICAM-1),CD44 and NF-КBP65 in bronchi epithelial cells of a rat model of asthma. Observe the anti-inflammation and airway hyperreactive effects of oxymatrine, a traditional Chinese medicine, as well as investigate the anti-asthmatic mechanism of oxymatrine.Methods: An animal model of asthma was established by ovalbumin (OVA) sensitizing-challenging SD rats. Forty eight rats were randomly divided into six groups: the control group, the asthma model group, the dexamethasone treating group and Oxy low dose ,midest dose, high dose treating groups. The anti-asthmatic effects were assessed by means of hemomyelogram of peripheral blood and bronchoalveolar lavage fluid (BALF), and histopathological examination. The lung outflow volume in vivo and the contractile response of trache-spiral-strip ex vivo to tacetylcholine(Ach) were detected through PowerLab system. Immunohistichemical technique was used to estimate the changes of ICAM-1,CD44 and NF-КBP65 in the rat bronchi epithelial cells.Results: (1) Differential count of peripheral blood leucocyte: The count of EOS of asthma group(10.5±1.9)was signifiantly higher than that of control group(2.5±0.5)(P<0.01),compared to asthma group, the lumbers of EOS of dexamethasone treating group and Oxy treating groups were dramaticly depressed(P<0.01);Compared to control group and dexamethasone treating group, the lumber of EOS of Oxy high dose treating group had no difference(P>0.05).(2) Differential count of BALF: Compared to control group, the lumber of EOS of asthma group was significantly increased(P<0.01). The lumbers of EOS of dexamethasone treating group, Oxy midest and high dose treating groups were significant lower than that of astham group(P<0.01). The rusults indicate that with the dose of Oxy enhanced, the potency of decreasing the EOS infiltrating to airway was more obvious.(3) The effect of Oxy on airway hyperresponsiveness: The contractile response of trache-spiral-strip to Ach were lower in dexamethasone treating group, Oxy midest and high dose treating groups were obviously decreased than that of asthma group(P<0.01); Compared to asthma group, changes of lung outflow volume of all medicine treating groups were obviously decreased(P<0.01),and that of Oxy high dose treating group had no difference with dexamethasone treating group(P>0.05).(4) The pathomorphology examination of lung tissue: In asthma group, we could find that the membrana mucosa plica became multiple, interalveolar septum thickening and the thickness of airway smooth muscle increased. A great quantity of EOS infiltrated to membrana mucosa, bronchi wall and pulmonum interstitium lveoli. Inflammatory reactions of dexamethasone treating group and Oxy treating groups were obviously extenuated.(5) The immunohistochemistry stain results displayed that the expression of ICAM-1,CD44 and NF-КBP65 in bronchi epithelial cells of asthma group was significantly higher than that other groups(P<0.01); After treated with Oxy, expression of ICAM-1,CD44 and NF-КBP65 was drimaticly decreased , the Oxy high dose treating group was more obvious than low and modest dose treating groups.Conclusion: Oxy can decrease the expressions of ICAM-1,CD44 and NF-КBP65 in bronchi epithelial cells of asthmatic rat , extenuate inflammation cells especially EOS infiltration to airway tissue, relieve inflammation reaction and depress airway hyperresponsiveness.
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