The Relationship Of TGF-β₁,Interleukin-13,VEGF In The Airway Of Asthmatic Guinea Pigs And Huang Qi's Effection

Objective To investigate the change of TGF-β1,IL-13,VEGF in the the airway of asthmatic guinea pigs.more importantly,to study the effects of Huang Qi on TGF-β1,Interleukin-13,VEGF in the the airway of asthmatic guinea pigs and explore the possible therapeutic mechanism of Huang Qi on asthma .Methods Forty adult male guinea pigs were randomly divided into control group (group A),asthmatic group (group B), arsenic huang qi group (group C) and dexamethasone group(group D),ten in each group. Asthmatic airway remodeling model was established by ovalbumin intraperitoneal combined with inhalation . The guinea pigs were killed at 24 h after the last challenge . Use the image analysis device to analysis the information of the airway remodeling. Immunohistochemistry detected the expression of TGF-β1,Interleukin-13,VEGF in the lung tissue.Results The main cause of airway remodeling is hyperplasia of airway smooth muscle,Huangqi can suppress the airway remodeling by restrain the hyperplasia of airway smooth muscle. The expression of TGF-β1,IL-13,VEGF in lung of group B were significantly higher than that in groupA;compared with group B,there were significant decrease in group C and group D. the differences were significant (P < 0.01) .Conclusions1. Expression of TGF-β1,IL-13,VEGF in the airway of guinea pig with asthma were remarkably increased.2. Huang qi may achieve its anti-inflammatory mechanism based on reducing the production of TGF-β1,IL-13,VEGF in the airway.