Effect Of Recombinant Human Interferon-γ On The Sca Fibroblasts From Rabbit Cleft Palate Bone Denudation

Objective: To study the effects of recombinant human interferon–γ(rhIFN–γ) on the proliferation of bone denudation scar fibroblasts after cleft palate surgery in vitro, in order to observe the biological effects of rhIFN–γon bone denudation scar fibroblasts and to provide the experiment basis for applying rhIFN–γinto the prevention and treatment of bone denudation scar after cleft palate surgery.Method:Establish the rabbit model of bone denudation after cleft palate surgery. Bone denudation scar fibroblasts were cultivated in vitro. rhIFN–γwas added to the cultivated hypertrophic scar fibroblasts. MTT assay was used to test the effects of rhIFN–γon proliferation of scar fibroblasts. Flow cytometry was also employed to measure the influence of rhIFN–γto the biological behavior of the palate scar fibroblast cell division cycle and the pathological changes. SPSS 13.0 software package was used to analyze the data, with P<0.05 for having significant difference。Result:1. Add rhIFN–γof different concentration to bone denudation scar fibroblasts after cleft palate surgery in vitro. The result was tested by using MTT assay.: Compared with the control group, the cell population of the experimental group was obviously less than that of the control group. It indicated that when the concentration of rhIFN–γreaches a certain level, it will inhibit the division growth of bone denudation scar fibroblasts after cleft palate surgery. In addition, it is the dose-response relationship between rhIFN–γand the change of cell population.2. The result was tested by employing flow cytometry to measure the fibroblast cycle: With the increase of the concentration of rhIFN–γ, the cell population decreased accordingly at DNA synthesis phase and postsynthetic stage, while the cell population increased at presynthesis phase.Conclusion: RhIFN–γis effective in controlling DNA synthesis and proliferation of bone denudation scar fibroblasts after cleft palate surgery, thus to inhibit the synthesis and proliferation of fibroblasts, suggesting that rhIFN–γis the negative regulator of fibroblasts. It provided a basis for the prevention and treatment of bone denudation scar of living persons after cleft palate surgery, and it has a certain value in the formation of bone denudation scar after cleft palate surgery.