| Gastric cancer is one of the most common malignant tumor, and the incidence of gastric cancer is the highest in china. About 170,000 people die from gastric cancer Each year, and the number is almost close to quarter of all the deaths for tumor. More than 20,000 new patients with gastric cancer have come out every year. It is indeed a serious disease threat to people's health. RNA interference is a cellular pathway of gene silencing in a sequence-specific manner at the messenger RNA level. In order to explore the safe and effective method of gene therapy for gastric cance, three kinds of plasmid expression vectors corresponding to the endogenous Bcl-2, C-Raf and Bcl-2/C-Raf genes respectively were constructed, and then were separately transfected into three groups of colon cancer cell line BGC-823. For the three groups, the expression of mRNA and protein related to the target genes were studied, the cell activity was assessed, and the effect of RNAi on proliferation of the cells was determined. The results indicated the expression of mRNA and protein related to the target genes decreased in all three groups for the transfection of the specific interference plasmid. The cell activity in the group transfected with the two targets interference plasmid was lower than the other two groups transfected with only single target plasmid. For the groups transfected with interference plasmids targeting to Bcl-2, C-Raf and Bcl-2/C-Raf genes, the inhibition rates of RNAi on the proliferation of the BGC-823 cells are 43.87%, 40.64% and 63.85% respectively. The results indicated plasmid targeting to Bcl-2/C-Raf simultaneously can more significantly inhibit the proliferation of the gastric cancer cells, providing a theoretical basis to gastric cancer gene therapy.
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