Studies On The Chemical Constituents Of Alisma Orientalis (Sam.). Juzep

The Alisma genus is a genus of plants in the Alismataceae family. There are 10 species all over the world and 3 species in China,i.e.Alisma canaliculatum,Alisma gramineum,and Alisma orientale.Alisma orientale is widely cultivated in China and Japan.Its dried rhizome,as a crude drug,has been included in Pharmacopoeia of China and been prescribed for diuretic and Damp-heat removal purposes in the treatment for dysuria,edema,distention,diarrhea,oliguria,retention of phlegm and fluid,infection of urinary system and hyperlipemia.The compounds isolated from Alisma orientale are mainly terpanes including tetracyclic triterpene and sesquiterpenes,besides fatty acid,phytosterol and other compounds.The current pharmacology studies have shown that Alisma orientale is useful in the therapy of atheroma,hyperlipemia,diuretics, hypertension,fatty liver and so on.The chemical constituent of the tuber of Alisma orientale was studied.The ethanol extract of the tuber were isolated by means of silica gel column chromatography(CC) and Sephadex LH-20 gel column chromatography,preparative thin layer chromatography(PTLC) and recrystallization etc.The structures of the twenty-four isolated compounds were elucidated by spectroscopic methods(IR,EI-MS, HR-ESI-MS,¹HNMR,¹³CNMR,DEPT,¹H-¹HCOSY,HMBC,HSQC etc.).Most of the twenty-four isolated compounds were identified as sesquiterpenes and triterpenes,and two of them were isolated as natural compounds first time.Finally,previous studies on the chemical constituents and the bioactivities of Alisma orientale were reviewed in this thesis.The results of this project were expected to enrich the database of natural products,to strengthen a significant scientific basis for chemotaxonomy,screening of new medicines and biosynthesis.