| Obesity is a chronic diseases that it can hazard to human health,and also be a key risk factor of most of disorders.In《Huang Di Nei Jing》it is associated with the levels of QI and blood as well as phlegm turbidity and blood stagnation in the body.Paeonia lacliflora Pall of Traditional Chinese medicine could be nourishing and activating-blood,pain Rougan,Yin received convergence Khan.it was belonged to the Yin medicine.Peoniflorin is the main biologically active ingredients of Peonia lacliflora Pall,modern medicine has a view that it possesses a variety of pharmacological activities.What we has done in vivo experiments have showned White peony Alba of decoction could inhibit the body weight of ablating the bilateral ovaries of the obese rats,and decrease the level of serum triglyceride (DG).At the same time,it could improve lipid metabolism disorder,remove too much the free radicals from body,and improve the antioxidant capacity.However,there were some reports that the Peoniflorin has a characteristics with concentration-dependent functions. Therefore,this experiment could be done to observe the effection of peoniflorin on releasing of free fatty acids(FFA) and anti-oxidative capacity in ex vivo adipose tissue of rats with different concentrations of peoniflorin,and to explore its preliminary mechanism.Some healthy adult SD rats were selected,with weighting 250±20g.there were eight groups.①The normal control group:the Krebs liquid contains Sodium Chloride(0.9%);②The Adrenaline Hydrochloride group:it contains Adrenaline Hydrochlaride Injection (10-6mol/L);③The high dose of peoniflorin group:the Krebs liquid contains Peoniflorin of 10-5mol/L;④The medium dose of peoniflorin group:the Krebs liquid contains Peoniflorin of 10-6 mol/L;⑤The low dose of peoniflorin group:the Krebs liquid contains Peoniflorin of 10-7 mol/L;⑥The Propranolol(10-5mol/L) + peoniflorin(10-6mol/L) groups: Propranolol(10-6mol/L) were put into the Krebs liquid which contains of peoniflorin (10-6mol/L);⑦The varapamil(10-5mol/L)+peoniflorin(10-6mol/L) group:Varapamil (10-5mol/L) were put into the Krebs liquid which contains of peoniflorin(10-6 mol/L);⑧The adenylate cyclase inhibitor(10-5mol/L)+peoniflorin(10-6mol/L) group:SQ22,536 (10-6mol/L) were put into the Krebs liquid which contains of peoniflorin(10-6mol/L).These healthy adult SD rats were luxatio to death.Some retroperitoneal adipose tissue of there rats were selected and divided into each block for 50±2.0mg,then,incubated them into Krebs liquid which contains bovine serum albumin(BSA,0.04g/mL) and the paeoniflorin (10-5 mol/L,10-6mol/L,10-7mol/L),Propranolol,varapamil and SQ22,536(10-6 mol/L) for 90 minutes,and the dose of these drugs is 20μL.In this course of incubating,continued to ventilate mixed gas of 95%O2 and 5%CO2.Finally,drawing some liquid to measure the contents of free fatty acids(FFA),malondialdehyde(MDA),total anti-oxidative capacity (T-AOC) with partly colorimetry,and tumor necrosis factor-a(TNF-a),interleukin-6 (IL-6),Leptin with enzyme-linked immunosorbent assay(ELISA).Statistics is dealed with statistical software of SPSS11.5,the results are indicated with mean±standard deviation((?)±s).Amang the groups significant difference were showed by single-factor analysis of variance.The meanling results were considered when p＜0.05 that the difference is statistically significant.The results are as follows:1.The peoniflorin and these blocking agents effected on the dilivery of free fatty acids from the adipose tissue:It was discovered that the peoniflorin(10-5mol/L,10-6mol/L) which compared with the normal group not only could respectively promote the dilivery of free fatty acids from the adipose tissue,but also there was a dose-related relation between peoniflorin and the dilivery of free fatty acids.However,peoniflorin(10-7 mol/L) has been not this effective action.2.The peoniflorin(10-6mol/L) and these blocking agents effected on the dilivery of free fatty acids from the adipose tissue:When Propranolol,verapamil and adenylate cyclase inhibitor(ACI,SQ22,536) were respectively mingled with peoniflorin(10-6 mol/L),the promotive effection of peoniflorin on dilivery of free fatty acids in ex vivo adipose tissue could be partially inhibited by these blocking agents.3.The peoniflorin(10-6mol/L) and these blocking agents effected on the dilivery of malondialdehyde and total anti-oxidative capacity from the adipose tissue:The peoniflorin(10-6mol/L) could decrease the quantity contained of malondialdehyde and increase the quantity contained of total anti-oxidative capacity.This effection could be infuenced by Propranolol,varapamil and SQ22,536.4.The peoniflorin(10-6mol/L) and these blocking agents effected on the release of some cytokines from the adipose cell:Compared the peoniflorin(10-6mol/L) group with the normal group,there were not only statictical significant changes(P＞0.05)of leptin,tumor necrosis factor-a and interleukin-6;when the peoniflorin(10-6mol/L) was apart put into the Propranolol(10-5 mol/L),varapamil(10-5mol/L) and SQ22,536(10-5mol/L),but also,there were not statictical significant changes,compared with the the peoniflorin(10-6mol/L).The consequenc suggested that the mechanism of which peoniflorin influenced on releasing of free fatty acids,decreased the contents of malondialdehyde and increased the contents of total anti-oxidative capacity in ex vivo adipose tissue of rats may be partly mediated by adrenergicβ-receptor,verapamil-sensitive L-type Ca2+ channel and signal transductive pathway via the second messenger cyclic adenosine monophosphate(cAMP). Therefore,It was speculated that peoniflorin could improve the anti-oxidative capacity of the organism by clearing away the excess free radicals,but also there was a dose-related relation between peoniflorin and the dilivery of free fatty acids.Bsides,peoniflorin was not immediately effect on leptin,tumor necrosis factor-a and interleukin-6.
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