The Expression Of KAI1/CD82 And Integrin β1 And Their Relationship In Endometrial Carcinoma

Objective:The purpose of the study is to examine the expression of KAI1/CD82 protein and Integrinβ1 in women with endometrial carcinoma,and investigate the relationship between them and the clinicopathological parameters.We studied important theoretical and clinical meanings in the happening,invasion and metastasis of endometrial carcinoma.Methods:In this study,we random select 46 samples of endometrial carcinoma,14 samples of atypical hyperplasia endometrial tissue,14 samples of normal proliferative phase endometrial tissue.All patients had abdominal hysterectomy in the department of Gynecology in the second hospital of Tianjin Medical University from January 2002 to June 2008.All these samples are proved adenocarcinoma by pathology. Primary endometrial carcinoma tissue,atypical hyperplasia endometrial tissue and normal proliferative phase endometrial tissue were examined in this study.These tissues were stained by S-P method of Immunohistochemistry to detect the expressions of KAI1/CD82 and Integrinβ1.Results:The positive expression rate of KAI1/CD82 in endometrial carcinoma (45.7%) was significantly lower than that in normal proliferative phase endometrium (92.3%),and uterine atypical endometrial hyperplasia(78.6%),(P<0.01,P<0.05). But the difference between uterine atypical endometrial hyperplasia and normal proliferative phase endometrium was not significant(P > 0.05).The positive expression rate of KAI1/CD82 showed a significant relation with FIGO stage, histological grade,myometrium invasion depth and lymph node metastasis in endometrial adenocarcinoma.The expression of KAI1/CD82 in late clinical stage(ⅡtoⅢ)(26.1%) was significantly lower than that in primary clinical stage(65.2%). The expression of KAI1/CD82 in low and second histological grades was distinctly lower than that in high histological grades(P<0.05),but the difference between low histological grades and second histological grades was not significant(P>0.05).The expression of KAI1/CD82 with deep invasion(>1/2)(27.3%) was markedly lower than that without or with superficial invasion(≤1/2)(62.5%),(P=0.017).The expression of KAI1/CD82 with lymph metastasis(18.2%) was statisticaly lower than that without lymph metastasis(54.3%),(P=0.005).The abnormal expression rate of Integrinβ1 in endometrial carcinoma(58.7%) were significantly higher than atypical endometrial hyperplasia(21.4%) and normal proliferative phase endometrium(7.7%), (P<0.05,P<0.001).But the difference between atypical endometrial hyperplasia and normal proliferative phase endometrium was not significant(P>0.05).The positive expression rate of Integrinβ1 showed a significant relation with histological grade and lymph node metastasis in endometrial adenocarcinoma.The expression of Integrin(51 in high and second histological grades was distinctly lower than that in low histological grades(P<0.05).The positive expression rate of Integrinβ1 with lymph metastasis(90.9%) was significantly higher than that without lymph metastasis (40.0%),(P=0.003).There was a significant relationship between low expression of KAI1/CD82 and over expression of Integrinβ1 in endometrial carcinoma.(r=-0.433, P=0.003).Conclusions:Compared to normal proliferative phase endometrium and atypical endometrial hyperplasia,the expression of KAI1/CD82 was reduced in endometrial carcinoma.Abnormal expression rate of KAI1/CD82 was significant relation with FIGO stage,histological grade,myometrium invasion depth and lymph node metastasis.The expression of KAI1/CD82 might be used as an objective indicator of the metastatic potency of local lymph node,extent of invasion and the prognosis in endometrial carcinoma.The positive expression rate of Integrinβ1 in endometrial carcinoma was much higer than normal proliferative phase endometrium and atypical endometrial hyperplasia.Integrinβ1 might be valuable markers in assessing biological behavior,invasion and metastatic potential and prognosis in endometrial carcinoma. Positive correlation was found between low expression of KAI1/CD82 and high expression of Integrinβ1 in endometrial carcinoma.Evaluating both of their positive rates might be useful to tumor diagnosis,estimating potency of invasion and metastasis,evaluating the prognosis of endometrial carcinoma and guiding the reasonable comprehensive treatment.