| Objective:In order to study the effects of TNFα-TNFR system in glaucomatousRGCs damage,the expression of TNFR1 and TNFR2 was observed in the retina of chronic elevated IOP rat models.Methods:1.Rats were rendered elevated intraocular pressure by ligating 3 episcleral veins along with subconjunctival injection of 5-Fu.2.The different time-point expressions of TNFR1 and TNFR2 in retina after elevation of IOP were analyzed by Western Blot.3.The single-expressions of TNFR1 and TNFR2 and the co-expressions of TNFR1-NF200,TNFR 1-GFAP,TNFR 1 -0X42,TNFR2-NF200,TNFR2-GFAP,TNFR2-OX42 were analyzed by immuno-fluorescence on cryostat sections of the operative groups and the control group.4.The co-expression of TNFR2-NF200 was analyzed by immuno-fluorescence on the whole retinal mounts of the operative group and the control group.Results:1.Elevated IOP was consistently maintained for 28d in experimental group. However the long-term model achievement ratio and stability-still need to be enhanced.2.The expressions of TNFR1 and TNFR2 were both up-reglulated after the elevation of IOP.The reaction of TNFR2 was more sensitive to elevated IOP than TNFR1.which began to increase early in 3days after operation.The co-expression of TNFR1-GFAP,TNFR2-NF200,TNFR2-GFAP and TNFR2-OX42 had been deteced in the retina 14 days after the elevation of IOP.3.The up-regulation of TNFR2 occurred mainly in the smaller RGCs,rather than in the larger or intermediate RGCs 14 days after the elevation of IOP.Conclusions:In the chronic elevated IOP rat models,the expression of these two types of TNFR in different retinal layers or different types of retinal cells possibly influence the final effects of TNF-αin glaucoma. |
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