Mechanism Of Transcriptional Regulation Of The HEY1 Gene In Prostate Cancer Cells

HEY1 is an important transcription corepressor in the formation of vascular endothelial cells,and it is a member of HEY gene family which participates in the formation of cardiac valves,development of cardiac muscle and aorta. What is more,it can influence heart development by mediating Notch signaling pathway. HEY1 is the direct target gene of the Notch signal pathway, whereas Notch signaling pathway has a significant role in generation and development of tumor. The activation of Notch1 can result in growth inhibition of prostate cancer cell. However, the transcriptional regulation mechanism and influence on prostate cancer cell of HEY1 gene remain to be discovered.According to the experiment, we found that the expression of HEY1 had a significant negative correlation in two prostate cancer cells of distinct grade malignancy—LNCaP and DU145. And interference of the PcG protein components EZH2,SUZ12,BMI1 promoted the mRNA and protein level of HEY1 gene, indicating PcG protein, especially SUZ12 can regulate the transcription of HEY1 gene; The result of RT-PCR, Real time PCR and Western blot showed that anti-cancer drug DNA methylase inhibitor 5-aza-dc could remarkably promote the mRNA and protein level of HEY1 gene, indicating that DNA methylation might participate in the regulation of HEY1 gene; We overexpressed HEY1 gene in DU145, and the result showed that the cell growth arrested in the G1 phase, indicating HEY1 gene was closely related to the cell cycle.The study briefly exploited transcriptional regulation mechanism of HEY1 gene, laying a good foundation to deeply further study the role of HEY1 gene in generation and development in prostate cancer cells.