The Investment On The Relationship Between Fetal Birth Weight And The Activity Of P⁷⁰S6K And Their Signal Transmission

Objective1.To investigate the relationship between the birth weight of fetus and the activities of P⁷⁰S6K.2.To investigate whether insulin and rapamycin can affect the activities of P⁷⁰S6K by the mTOR/P⁷⁰S6K signaling pathway in the cells of the placental tissues.Methods70 cases of mature placental tissues after uterine-incision delivery without complications(such as hypertension,diabetes,heart disease,endocrine disturbance,fat metabolism abnormality) in the gestational period were selected as the investigated subjects,body mass index of the progestation is normal(20≤BMI≤24.0),body weight gain in the duration of pregnancy is alike.(except abnormal placenta and umbilical)To investigate the relationship between the birth weight of fetus and the activities of P⁷⁰S6K.they were divided into three groups:â‘ group1(the weigh of the fetus≥4000g)â‘¡group2(3000g≤the weight of the fetus<4000g)â‘¢group3(the weight of the fetus<3000g) according to their birth weights,there are 20 cases in the every groupTo investigate whether insulin and rapamycin can affect the activities of P⁷⁰S6K by the mTOR/P⁷⁰S6K signaling pathway in the cells of the placental tissues that were divided into three group according to stimulating factors. â‘ insulin groupâ‘¡rapamycin groupâ‘¢control group There are 10 cases in the every group。Activities of P⁷⁰S6K were carried out by immunoprecipitation,incoparation of [r-³²P]ATP,tissue cell culture.Results1.There were significant statistical differences in the comparison of P⁷⁰S6K activities,group1>group2>group3,(group1,2:P<0.05,group2,3:P<0.05 group 1,3: P<0.01).2.The P⁷⁰S6K activities in the placenta from the insulin stimulation group was significantly elevated when compared with that of the control group,and the statistical significance was found in the difference(P<0.01);P⁷⁰S6K activities in the placenta in the rapamycin group and it was statistical significant when compared with the insulin group(P<0.01).Conclusion1.The activities of P⁷⁰S6K protein in the placental tissues increased with the increase in the birth weight of the fetus and the activities of P⁷⁰S6K was also regulated by the mTOR/P⁷⁰S6K signaling pathway,indicating that there was certain relationship between the mTOR/P⁷⁰S6K signaling pathway in the placental tissues and the birth weight of the fetus.2.Insulin and rapamycin can regulate the activities of P⁷⁰S6K in the placenta,indi-cating that insulin and rapamycin can regulate the activities of P⁷⁰S6K in the placenta by the mTOR/P⁷⁰S6K signaling transduction pathway and insulin regulate birth weight completely or partly and further affect the birth weight of the fetus.