| ObjectiveUrticaria is the skin and mucous membrane disease that is caused by multiple factors,the key feature of Urticaria are edema wheal stoking for many time,feeling pruritus in self-consciouseness,progress prolonging above six weeks,even causing Throat mucous membrane that endanger life.because of complicated etiological factors and longer progress,many time breaking out,difficult prevention and bad treatment effect of routing Antihistamine,study of the pathogenesis,prevention and cure on chronic urticaria has become important topic in medical science kindom.Investigation of modern medicine detect:function diseqilibrium of T Helper cell sudgroup(Th1 and Th2)and chronic urticaria has close correlation.Secretory cytokine level of CU patient PBMC decreases,but lecel of Th2 cytokine steps up.After treatment Th1 cytokine and Th2 cytokine trends to equilibration,therefoer chronic urticaria is a skin immune inflammatory reaction that Th2 cells hold main status.Traditional medicine reserves effective prescriptions to therapy chronic urticaria,but since many years the investigations to aim directly at it has only limited to clinical observation and profound experiment invesgations are absenct.The experiment used classical prescriptions from traditional medicine that were used to threapy urticaria-Xiao feng San--and the experiment investgated the effect of secretory Th1 and Th2 cytokines from PBMC of wind-head urticaria patients foundations were going to provided to the classical prescription- Xiao feng San -to therapy urticaria.Methods1 selective standard of acute urticaria patients.1.1 The surface of skin and mucous membrance occueerd recurrent xheals and the duration was not beyond twenty-four hours.1.2 The incidence could not be under two times weekly.1.3 The progress was not over six weeks.1.4 The antihistamine drugs was not used in one week and the steroid hormone and the immunosupppression drug were not uesd in one month.2 Exclusive standard of acute urticaria patients2.1 The physical urticatia was excluding because of the coldness and the pressure and so on.2.2 The autoimmunitydisease was excluding.3 The standard of syndrome differentiation3.1 Syndrome of exterior attacked by wind-heat:the color of wheal is bright red,the patient fell hot and very itching,to accompany with fervescence,nephrasthenia asthma,Swelling of throat,the skin rash will aggravate when the patient in the hot environment,thin lingual fur and white or yellow,pulses is floating and quick. 3.2 Syndrome of exterior tightened by wind-cold Syndrome of exterior attacked by wind-heat:the color of wheal is white,the skin rash will aggravate when the patient meet wind -cold and anesis in the hot environment,hydroadipsia,texture of tongue is light,coated tongue is white,pulses is floating and tight.3.3 Syndrome of hemo penia wind dryness:recurrent attacks, protraction for long time,the skin rash will aggravate in the post meridiem or at night,to accompany with upset and tantrum,dry mouth,feverish sensation over the palm and sole, red tongue hyp- bodyfluid,pulses is deep and trifling.4 Monocytic seperated technique in venous blood:there are 15 petients of acute ueticaria and fresh venousblood was going to be drawn from every patients.In order to obtan Lymphocytes and Monocyts the Ficoll iso-sedimentation seperation was used and the cell susqension was produced whir RMPI1640 and the celluar tele-concengtration was 1×106/ml.5 Detdcting method of cellular vital force:the teypan blue execlusion test wasselected and after stanging with 0.4%trypan blue counted vital cell could be above 95%in three minutes.6 Routine obseration of cultured cell.6.1 The changes of form and tiny formation:the volum of lymphoblasts largened and the nuclei were transparent and ball agglutiphenomenon occurrded.6.2 Observation of culture medium:the normal fresh culture medium containing phenolsulfonphthalein was pewchblow and as so as it became primary colors and the possibility of bacteria contamination needed be attented.7 The cuitivation of pro-generation cell:the cells were coltured in vitro with RPMI1640 culture medium in the 96 cell culture plates and had been hatched for 48 hours in the carbon dioxid incubator on the condition of 37℃,and then had been centrifugated by mesns of 2000r/min,at last the surperior-schedul liquor were collected and were preserved on the condition of -20℃.8 The drtecting method:the IL-4 and IFN-γcontents from the surerior-schedul liquor of every group were detected by means of ELISA.ResultThe change to the form and the tiny constitution:after being stimulated by the PHA,T cells occoured a series of proliferation response as cells velum largening and the nucleus distinctnees and cyloplasmic manifold and the vacuole occurred and the chromatin rarefaction and the ball coagulation phenomenon,all of this proved that the lymphocyte had translated into the lymphocytoblastThe location of PHA best concentration:Different concentrations of PHA stimulated lymphocytes,respectively, after 48 hours,according to the volume of INF-γsecretion was found in the change in peak 10ug/ml around,but in order to ensure that the stability of INF-γsecretion in thestability, PHA stimulation of the concentration of the final choice 8 ug / ml.Wind-heat mononuclear cells on the impact of value-added: synergy in the stimulation of PHA,the extinction of various concentrations of the role of the wind,scatteredmononuclear cells in the processing of the OD492 values were higher than the control group,and the concentration of 10-3g/ml～10-6g/ml significant difference when both(P＜0.05). Wind-heat and the supernatant of PBMC in IL-4,IFN-γsecretion of effects:compared with blank control group,by adding various concentrations of Wind-scattered and content after the IFN-γincreased in varying degrees,and the medicine concentration in IFN-γwhen 10-5g/ml content reached its peak, adding 10-4,10-5 g / ml solution extinction when the wind scattered the contents of IFN-γhavea significant difference (P＜0.05).IL-4 levels declined,but no significant difference (P＞0.05).
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