Study On The Gene Therapy For Severe Traumatic Brain Injury Through A20

Background:Apoptosis is one of the most important means of cell-death after traumatic brain injury(TBI),and then how to suppress apoptosis to maintain more neurocyte is one of the focus on the study of TBI.A20 is able to suppress the apoptosis induced by TNFR.We increase the expression of A20 after TBI,in the aim of analysis on the effect of gene therapy for head trauma through A20.Method:Sprague-Dawley rats were assigned randomly to study group and control group(35 rats every group).After severe TBI,the rats in study group were injected liposome-pcDNA3.1-A20,and those in control group were injected liposome pcDNA3.1-A20.In each group animals are sacrificed 6h,24h,72h,168h after operation. The expression of A20 and neurocyte apoptosis rate are defined by immunohistological method and TUNEL accordingly.The remaining animals were testified for the neurological function.Results:The expression of A20 was higher than control group with statistical differences.The peak of apoptosis of neurocyte was found at the time of 72h after TBI, and at the time of 12h,24h,48h,72h,the number of apoptosis in the study group was lower than control group.At the time of 4w after TBI,the rats' neurological function in the study group was better than control group. Conclusion:Gene therapy for head trauma through A20 may have the effect of anti-apoptosis,and improve the outcome after TBI.