Detection Of Active Human Cytomegalovirus Infection In Bone Marrow And Hematopoietic Stem Cell Transplant Recipients

Background:Human cytomegalovirus(HCMV)is one of the most important causes of morbidity and mortality in bone marrow and hematopoietic stem cell transplant recipients. Sensitive,specific and timely diagnostic tests for the detection of HCMV infection and activation remain essential for the success of transplant therapy.Methods and Objectives:In this study,528 samples of whole blood were collected from 28 bone marrow and hematopoietic stem cell transplant recipients.The results of pp65 and IE antigens by immunocytochemical staining and HCMV IgM by enzyme-linked immunosorbent assays(ELISA)were analyzed,which determined the diagnostic value of Active HCMV infection.Results:In 528 samples of whole blood,the positive rates of pp65 and IE antigenaemia were 70.8%and 71.2%,respectively.The coincidence level was 80.1%(p=0.921).The levels of HCMV detected by the pp65 and IE antigenaemia assays were significantly correlated(R=0.775).The first checkout time of pp65 and IE antigenaemia after transplantation were 15.61±12.52,17.04±15.92 days,respectively(P=0.710).Moreover, 11.6%of HCMV IgM was positive in 528 samples.The coincidence level of IgM and pp65,IE antigenaemia were 36.7%and 35.0%,respectively(p＜0.05).Conclusions:Therefore,IE antigen by immunocytochemical staining could a useful parameter of detecting active HCMV infection in bone marrow and hematopoietic stem cell transplant recipients,but HCMV IgM was not suitable for the diagnose.