Experimental Study On The Change Of Intestinal Immune Function And Glutamine Metabolism In The Intestinal Mucosa In Rats With Hyperlipidemia And Acute Necrotizing Pancreatitis

Objective: To build a model of rats suffered from hyperlipemia (HL)and acute necrotic pancreatitis(ANP). To observe the change of intestinal immune function and glutamine metabolism in the intestinal mucosa in ANP in rats with and without HL,and to explore the relationship between the change of intestinal immune function and glutamine metabolism in the intestinal mucosa in rats with HL and ANP to learn further the possible mechanism of the injury of intestinal immune function in ANP and the mechanism on intestinal immune function that HL aggravate the pathogenetic condition in ANP.Methods: 40 male SD rats were randomly divided into 4 groups: control group, HL group,ANP group,HL+ANP group.The specific methods are as follows :First,the rats were randomly divided into two groups(N=20) , respectively feeding normal diet and high-fat diet (which consist of 87.8%nomal diet , 10% lard ,2% cholesterol and 0.2%sodium cholate) for 4 weeks.After 4 weeks,each group was randomly devided into two groups(n=10) again and used retrograde injection 3.5% sodium taurocholate which induced severe acute pancreatitis and NS into the pancreatic duct respectively. Rats were sacrificed ,and the materials were gained at 24 hours after models were made. To observed the histopathologic damage degree of pancreas and measure the value of amylase (AMS),triglyceride (TG) in serum. Endotoxin concentration in portal vein was determined by limulus methods;CD4+, CD8+T lymphocytes in intestinal mucosa were examined by immunohistochemistry;the sIgA contents of intestinal mucosa was examined by radioimmunoassay;the glutamine uptake rate,concentration of glutamine and activity of GA in intestinal mucosa was examined by using spectrophotometry.Results: The serum TG value in the HL group was higher than that in the control group ( P < 0. 01) . .Compared to the control group ,the pancreas were found to get bleeding and necrosis histopathologic change,and the level of AMS in serum and plasma endotoxin concentration in the portal vein increased, and the levels of CD4+,CD8+T lymphocyte ,sIgA ,glutamine uptake rate,glutamine and activity of GA in the intestinal mucosa reduced significantly in the ANP group (P<0.01～P<0.05);Compared to the ANP group ,the HL+ANP group had more serious histopathologic change , higher levels of AMS and TG in serum and plasma endotoxin concentration in the portal vein ,and lower levels of CD4+,CD8+T lymphocyte , sIgA , glutamine uptake rate , glutamine and activity of GA in the intestinal mucosa(P<0.01～P<0.05).Conclusion: An ideal animal model.of HL can be builded by feeding high-fat diet for 4 weeks.A model of ANP complicated with hyperlipemia in rat might be developed by feeding high-fat diet for 4 weeks and retrograde injection of 3.5% sodium taurocholate into the pancreatic duct.Intestinal immune suppression occurred in the early stage of ANP,and the change of glutamine metabolism in the intestinal mucosa may play an important role here. HL can make ANP more serious through intestinal immune suppression way,and the change of glutamine metabolism in the intestinal mucosa may play an important role here.