The Effects And Mechanisms Of The Heat Shock Protein 70 (HSP70) Influence The Proliferation Of MCF-7

[Objective] To study the effects of the heat shock protein 70 (HSP70) how to influence MCF-7 which is the human breast cancer cells, and to explore it's primarily mechanisms. [Methods] MCF-7 cells were handled by different concentrations of Hsp70 for three days, and then detected the activation of the cells by cell counting and MTT assay; detected the expression of cyclinD1a and cyclinD1b which is the TLR signal transduction related genes by RT-PCR, detected the phosphorylation of I-κB and ERK1 / 2 which is the TLR signal pathway by Western Blot at 0 minutes, 5 minutes, 15 minutes, 30 minutes and 60 minutes after added HSP70. [Results] The cell counting and MTT tests of MCF-7 showed that the cell number and the OD value were significantly increased after added Hsp70, and the proliferation was significantly. MCF-7 cells dealt with Hsp70 after three days, the expression. of cyclinD1a and cyclinD1b by RT-PCR method.showed that the quality of cyclinD1a was significantly increased but without any amplified fragment of cyclinD1b. Detected the phosphorylation of I-κB and ERK1 / 2 by Western Blot,which showed after dealt with HSP70 for 5 minutes. the phosphorylation of I-κB, ERK1 / 2 began to increase, and the highest expression appeared after 30 minutes. [Conclusion] MCF-7 cells dealt with Hsp70 for 3 days, its biological characteristics such as cell proliferation had changed, and upregulated cyclinD1a which was the related TLR signal transduction signaling molecules, phosphorylated ERK1 / 2, and phosphorylated I-κB. Therefore,as a ligand of TLR ,HSP70 facilitated the genesis and development of tumor through TLR signal transduction pathway.