| Objective Observe the effects of self-assembled IKVAV peptide nanofibers on dorsal root ganglia neurons (DRGn) and DRG through co-culture of DRG and gel.Methods The peptide was dissolved in NaOH solution where PH was adjust to 8.5 and the content of peptide was 1wt%. 1wt% peptide covered with coverslip was triggered to be self-organized into gel by the addition of DMEM/F12, which was detected by TEM. The dorsal root ganglion (DRG) explants were collected from newborn rats, and then the tissues were dispersed into individual cell suspension by primary isolated culture methods. The DRG and dissociated cells were divided into experimental (EG) and control group (CG). In EG: DRG and cells were transplanted on the surface of gels. In control groups (CG): DRGn were transplanted on the surface of polylysine. Growth of DRGn was observed by IPCM. The gel's cytotoxicity and its effect on attachment of DRG neurons, DRGn's live time and the length of neurit in EG and CG were detected by live/dead assay kit and immunofluorescence staining combining with cell counting.Results TEM showed that the self-assembly gel was composed of network nanofibers dimensional culture system cell proliferation were better. The growth of neuritis from DRG in EG was better than it in CG. There were no significant difference in the percent of alive cells between EG and CG after 1d and 7d of culture. The number of attached DRG neurons in EG was higher than that in CG after 3h and 6h of culture. The length of neurite of DRGn in EG and CG is 43.8±10.4μm,33.4±5.75μm. The neuron percentage after 14 days in EG and CG is 43.60±4.83%,39.04±3.87%. Conclusion The gel self-organized from IKVAV-containing peptides, which can degrade the mortality of neurons and promote neurite sprouting and direct neurite growth and enhance the growth of DRG and attachment of DRG neurons, has low toxicity to cells, can be acted as a superordinary nerve tissue engineering scaffold. |
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