Research On The Protective Effect Of BFGF On Cisplatin-Induced Kidney Damage

Objectives: to investigate the renal toxicity in mice treated with cisplatin and study the protective effect and the mechanism of bFGF by establishing animal model with cisplatin(cDDP )induced kidney damage.Methods : thirty two male mice were randomly divided into four groups. : control group (0.9% saline solution), cisplatin (cDDP) group, cisplatin (cDDP)+low bFGF level group, and cisplatin (cDDP)+high bFGF level group. Their serum samples were collectd after injecting drugs for five days and The changes of Scr and BUN were measured. MDA level, SOD Activity, NO Content and activity of GSH-Px were tested in kidney homogenate.Result: (1) Compared with the control group, the Scr and BUN of the cDDP group was significantly elevated on the 5th day (P < 0. 01). (2) bFGF can reduce the damage which cause the increase of Scr , BUN. the content of MDA and NO decreased,while the activity of SOD and GSH-Px increased. (3) Histopathological analysis of the renal tubules in the cDDP group showed scattered areas of edema , degeneration. while cisplatin (cDDP)+bFGF group showed scattered areas of slight edema , degeneration and there was no obvious change in the renal tubules.Conclusion: (1)The animal models (mice) had cDDP (10mg/kg) induced stable kidney damage. (2)bFGF could reduce the damage which cause the increase of Scr , BUN and increase the activity of SOD and GSH-Px, thus it could protect the renal structure and function.