Changes In The Pathway Of Somatosensory Cortex And The Expression Of GFAP After Intracerebral Hemorrhage In Rats

Objective To investigate the changes in neural pathway of primary somatosensory and the expression of GFAP in the intracerebral hemorrhage in rats.Methods To set up an animal model of cerebral hemorrhage. A total of 20 experimental rats were randomly divided into two groups with 10 rats in each groups: control group and experimental group. A fluorogold (FG) retrograde tracing technique and Nissl were used to study the changes in the neural pathway of primary somatosensory. Immunohistochemical method was used to observe the expression of glial fibrillary acidic protein (GFAP) and the quantitative change of astrocytes in cerebral cortex, caudate nucleus, thalamus and brainstem.Results 1. Control group: The nerve cells of the rats were spherical and triangle, which ranked closely. Nissl body was dyed to royal blue. Experimental group: In the caudate nucleus, There can clearly see obviously hemorrhage region, where the number of nerve cells were decreased markedly compared with the control group. The pericaryon was spherical and ellipse. Nissl body was dyed to light blue. 2. Control group: The distribution of FG-labeled neurons was observed under fluorescent microscope. It was found that the FG-labeled Neurons were mainly distributed in the area of motor cortex (M1), somatosensory cortex ( S1,S2 ),insular cortex(GI, DI, AID, AIV, AIP), basal nucleus (Lss, LAcbsh, LGP, CeL, CeC, IPACL, IMG), cerebral ganglion ( VPM, VPL, PVP, LPMR, IMD, CL, MD, VPPC, Po, PrC). Experimental group: It was found that the FG-labeled Neurons were mainly distributed in the area of motor cortex (M1), somatosensory cortex (S1,S2), insular cortex (GI, DI), basal nucleus(LGP, IPACL), cerebral ganglion (VL, VM, VPL, VPM). The distribution range of the experimental groups is obviously smaller than the control groups. 3. The immunoreactivities of GFAP in all the cerebral cortex, caudate nucleus, thalamus and brainstem of experimental group were significantly enhanced compared with the control group Respectively (P<0.05).Conclusion The cerebral hemorrhage can cause partly block of sensory fiber conduction in encephalic region and activate the astrocyte to express GFAP in cerebral cortex, caudate nucleus, thalamus and brainstem. Our date suggestions that the changes in neural pathway of primary somatosensory and the expression of GFAP , which were important factors to cause disorder of intracerebral hemorrhage in rats. This maybe was a new mechanism.