The Effect Of Glucagons On Sclera Fibroblast Cell Of Guinea Pig In Vitro Culture

Objective we study the effect of glucagons on sclera fibroblast cell of guinea pig in vitro culture to approach the relationship between glucagons and myopia occurrence mechanism.Methods We choose healthy guinea pig of fifteen days randomly,then GSF were cultured in vitro and identified.The GSF of three to six generations in good condition were applied to medicine experiments.The time and concentration effect of glucagons on the proliferation of GSF were measured by using MTT,the effect of different concentrations of glucagons on the expression level of MMP-2 and TIMP-2 was measured by using Elisa method.Results Glucagons was related with the proliferation of GSF about time effect from the third day,it exists statistical difference between the two groups(P＜0.05).Glucagons was also related with the growth of GSF about dose effect in 10ng/ml-200ng/ml,it exists statistical difference between the two groups(P＜0.05).Glucagons have evident dose effect in 50ng/ml-200ng/ml,it exists significant statistical difference between the two groups(P＜0.01).Glucagons depress the expression of MMP-2(P＜0.05),the medicine have no obvious effect of TIMP-2.Conclusions Glucagons can promote the proliferation of GSF in vitro culture and depress the expression of MMP-2,the medicine may slow down the development the myopia on certain degree.