| Objective:The study was by detecting the expressions of PPARγ(Peroxisome proliferator-activated receptorγ,PPARγ) mRNA and protein levels and the activity of AP-1(activator protein-1,AP-1) which was downstream of PPARγin order to observe the protection of resveratrol in heart of SD rats feeding with high-fat diet.The positive control is pioglitazone which the ligands of PPARγ. The aims were to reveal the mechanisms that resveratol was being regulation the lipid metabolism,the antioxidant and the cardioprotection were able to by inhibiting the pathway of PPARγ/AP-1 activating in heart of high-fat diets rats.Methods:Four groups were randomly selected from 32 male Sprague-Dawley rats:control group(C), the hyperlipidemia group(HF high-fat diets),the pioglitazone positive group(PI high-fat diets) and the resveratrol positive group(RESV high-fat diets).At the same time every group were treated with 10mg/kg·d body weight pioglitazone,10mg/kg·d body weitgh resveratrol and distilled water(C and HF group) by gavage daily.At 0 week,3th week,6th week blood was gotten and the level of blood serum TG,TC,HDL-C were tested by kits.Rats were sacrificed at 6th week,blood was gotten from the abdominal aorta and the heart was taken to store at-70℃. TRIzol extracted the total RNA of heart and PPARγmRNA were measured by RT-PCR.The neucleoprotein were extracted by kits,the PPARγand AP-1 protein levels were detected by Western blotting.At 6th weeken,the content of lipid peroxidation production malonaldehyde(MDA), the activity of glutathion peroxidase(GPx) and superoxide dismutases(SOD) were detected by kits.Results:1.At 6th week,the levels of TG,TC in HF rats were higher than C rats which was shown that the models of hyperlipemia were established(P＜0.05).Compared with HF rats,the levels of TG,TC were reduced in HF+RESV rats(P＜0.05),but had no statistical difference between HF+PI and C(P＞0.05).2.At 6th week,compared with C rats,the contents of MDA were higher in HF rats,the activity of SOD and GPx were lower(P＜0.05).And compared with HF rats,MDA was lower reduced in HF rats(P＜0.05),SOD was more increased(P＜0.05).But the activity of GPx had no statistical significance compared to HF group(P＞0.05). 3.Compared with C rats,the expression of PPARγmRNA and protein levels were lower (P＜0.05).They were significantly increased in HF+RESV rats compared to HF group(P＜0.05), but had no statistical significance compared to HF+PI group(P＞0.05).4.Compared with C rats,the activity of AP-1 were higher in heart of HF rats(P＜0.05).AP-1 were significantly lower reduced in HF+RESV rats compared to HF rats(P＜0.05).Conclusions:1.Resveratrol increased the expression of PPARγmRNA and protein,attenuated TG and TC content in heart of high-fat diet rats.These data suggest that resveratrol regulates the lipid metabolism by activating PPARγ.Resveratrol also prevented the activity of AP-1 downstreaming signal transduction of PPARγ,reduced MDA content and increased the activity of SOD.These data demonstrate that there are exist associativity between AP-1 and oxidative stress in high-fat diet rats.2.Resveratrol increased PPARymRNA activity and decreased AP-1 activity in heart of high-fat diet rats.These data demonstrate that the mechanisms that resveratrol regulates the lipid metabolism and the antioxidant are possible by activating the pathway of PPARγ/AP-1.
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