The Effect Of Hepatocyte Growth Factor On Human Retinal Pigment Epithelial Cells Under High Glucose

Objective:To investigate the effects of Hepatocyte growth factor(HGF) on proliferation and the expression of vascuar endothelial growth factor(VEGF) and pigment epithelium derived factor(PEDF) of human retinal pigment epithelial(hRPE)cells cultured with high glucose in vitro.Methods:The hRPE cells were divided into normal glucose group(5.5 mmol/L glucose,), high glucose group(33 mmol/L glucose),high glucose plus HGF group(33 mmol/L glucose+ 50ng/ml HGF).12h,24h,48h,72h,96h later,the cell proliferation was observed by MTT assay.The hRPE were divided into normal glucose group(5.5 mmol/L glucose,NG),high glucose group(33 mmol/L glucose,HG),high glucose plus adiponectin group(33 mmol/L glucose plus 50ng/ml HGF).12h,24h,48h,72h,96h later,the level of VEGF and PEDF was measured by enzyme-linked immunosorbent assay(ELISA).Results:The treatment of hRPE cells with 33 mmol/L glucose caused a obvious decrease of celluar roliferation after 24h,48h,72h and 96h.Compared with high glucose group,HGF group after 12h,24h,detection of cell viability had no significant changes(P>0.05);48h,72h,96h later, cell viability had significant changes(P<0.05);Compared with normal glucose group,the VEGF level significantly increased and PEDF significantly decreased in high glucose group after24h,48h,72h and 96h.Compared with high glucose group,the VEGF level significantly increased and PEDF significantly decreased in high glucose plus HGF group(50ng/ml). (P<0.05).Conclusion:The high glucose inhibits the proliferation activity of hRPE and increase VEGF and decrease PEDF.50ng/ml HGF can promote the proliferation activity of hRPE and increase VEGF and decrease PEDF.HGF can regulate the balance of VEGF and PEDF to promote the formation of new blood vessels in diabetic retinopathy(DR).