The Low Dose-dependent Effect Of L-arginine In The Treatment Of Acute Pancreatitis In Rats.

ObjectiveThis study investigates the different small dose of L-arginine's effects in treating acute pancreatitis for experimental rats.MethodsAfter given different doses of L-arginine,to observe the serum amylase, the level of nitric oxide and the pathological changes of pancreatric tissues.1.Iodine-amidon chromatometry to test serum amylase.2.Nitrate reductase was used to determine the amount of NO2-/NO3- in pancreatic tissues and serum.3.Pancreatic tissue morphology was checked and a quantitative assessment were made about the pancreatic tissue injury.ResultsNO2-/NO3- content: AP group>L-arg treatment group(50,100,200mg/kg)> sham-operating group, AP group was higher than sham-operating group in serum amylase and pancreatic tissue pathology score, and it remains higher than sham-operating group even after L-arg treatment,but with the low-dose, the scope gradually reduced.ConclusionThe results suggest that acute pancreatitis, NO generation increased significantly. At given after L-Arg, and with the dose increase, NO concentration gradually reduced, combined with its pathological damage has reduced significantly and the serum amylase value decline on low-dose L-Arg on pancreatic protective effect, with the dose increased(50,100,200mg/kg) the effect became more obviously.