The Effects Of Preventing And Cure Of Cajanus Cajan(L.) Millsp On Rats Ovariectomized Induced To Postmenopausal Osteoporosis

Cajanus Cajan(L.) Millsp,with effects of invigorating the kidney, activating blood circulation and dissipating blood stasis,can promote new bone formation and promotion of angiogenesis the mechanism of which is the key to the treatment of bone disease.The Traditional Chinese Medicine deemde that postmenopausal osteoporosis is due to kidney deficiency and blood stasis predominantly.So basis to the idea of"definite the kind of syndrome with effective drugs,while syndrome and effects match",we belive that Cajanus Cajan(L.) Millsp can be used for prevention and treatment of the postmenopausal osteoporosis.This study examined the effects of Cajanus Cajan(L.) Millsp on bone mineral density and bone tissue morphology of ovariectomized rats.Select the 3-month-old female SD rats which were randomly divided into normal group,model group,Longmu Zhuanggu group,Cajanus Cajan(L.) Millsp high-dose group,Cajanus Cajan(L.) Millsp middle-dose group,Cajanus Cajan(L.) Millsp low-dose group.In addition to the normal group,other groups Establish postmenopausal osteoporosis mode through ovary resection of female rats. After 3 days,begin to delivery drugs in order to prevent and treat PMOP.The cycle eye mines blood on the eighth week of administration,RIA detection for ET levels in plasma(nuclear medicine laboratory),nitrate reductase assay for NO content in serum to explore for Cajanus Cajan(L.) Millsp the role of regulation vascular endothelial function.On the tenth week,in addition to Cajanus Cajan(L.) Millsp middle-dose group,all rats of groups else were detected whole body bone mineral density by dual-energy X(in Orthopedic hospital);ELISA assay to measure the serum VEGF levels,the left side femoral of 5 rats taken in each group to produce general pathology.The results are as follows:1,whole body bone density(BMD):The model group obvious reduced,compared to the normal group,which showed that making the model of rats PMOP through ovary resection is successful;Longmu Zhuanggu group,Cajanus Cajan(L.) Millsp high-dose group,Cajanus Cajan(L.) Millsp low-dose group are higher than the model group significantly,which can explained that Longmu Zhuanggu and Cajanus Cajan(L.) Millsp for rats with PMOP has the prevention and treatment function.2,bone pathology:Under the microscope observed bone trabeculae are full and the arrangement order of normal group rats,while model group bone trabeculae fracture,increased space between trabeculae,and even integration into cavity.Longmu Zhuanggu group bone trabeculae appear to be full but with an irregular arrangement,part of the region between the gap increased. Cajanus Cajan(L.) Millsp high and middle dose groups,low-dose group trabecular bone sparse.3,ET,NO and VEGF:Rats with PMOP ET elevates and NO drops,so the balance of ET/NO is destroyed.It leads to the formation of blood stasis.Longmu Zhuanggu group and Cajanus Cajan(L.) Millsp can promote the ET level,increase the NO content,inhibit the formation of blood stasis;The model group PMOP rats and normal group rats blood serum VEGF do not have the difference,but obviously delivering drugs groups are higher than model group respectively.From those,we infer that the mechanism of Longmu Zhuanggu group and Cajanus Cajan(L.) Millsp preventing and cure rats with PMOP is to regulate the balance of ET/NO,promote VEGF to express,advance the blood vessel to grow, increase the blood vessel permeability,ensure to supply enough nourishment for bone microcirculation and growth.This experiment proved that rats with PMOP show whole body bone density reduced,bone trabeculae sparse,ET/NO unbalanced,all of that correspond with the symdrom of kidney deficiency blood stasis of woman with PMOP.Longmu Zhuanggu group and Cajanus Cajan(L.) Millsp both can prevent and cure rats with PMOP effectively.It concerns to adjust the ET,NO,VEGF level possibly.