BTNL2 Polymorphism And Susceptibility To Tuberculosis In Chinese

Objective:To investigate the genetic polymorphisms of BTNL2 gene in Chinese,and to study the possible association of BTNL2 polymorphism and the susceptibility to tuberculosis.Methods:Blood samples from 96 tuberculosis cases and 96 controls were collected for a case-control study in Chinese.DNA of the subjects was extracted and amplified by polymerase chain reaction(PCR).The single nucleotide polymorphisms(SNPs) of BTNL2 were determined by SNaPshot SNP genotype techniques.The genotype and haplotype frequencies were compared between tuberculosis cases and controls using SHEsis software.Results:No association was detected between single SNP locus studied and disease. However,the C—A—T—G—C—G and C—G—T—G—C—G haplotype groups present a significant association with susceptibility to tuberculosis,with the OR(95%CI) to be 0.39(0.19～0.79) and 3.50(0.93～13.18) respectively.Conclusion:The single SNP in BTNL2 gene might not be associated with the susceptibility to tuberculosis in Chinese.But,the haplotype of BTNL2 gene may be associated with tuberculosis.