The Expressions Of PP2Ac In Various Types Lung Carcinomatous Tissues And Lung Carcinomatous Cells

Objective:To investigate the expressions and distribution of PP2Ac in human lung cancer and other different lung tissues and cells.Method:YTLMC-90,GLC-82 cell strains and normal lung tissue cells were cultured in laboratory.Adenocarcinoma,squamous-carcinoma and minicell -undifferentiated carcinoma,48 paraffin-embedded slices of this three ordinary types lung cancer were offered from the 1st affiliated hospital of Kunming medical university.Using the method of western blot and immunohistochemical staining to explore the expression of PP2Ac in them.Result:In this studies,the results of western blot demonstrate that the expression of PP2Ac in normal lung tissue cells was normal;and faint in YTLMC-90 cells;much faint and even disappeared in GLC-82 cells.In addition,the expression of p-AKT was low in normal lung tissue cells. OA,the inhibitor of PP2Ac,was given in GLC-82 cells,the findings was the expression of p-AKT enhanced obviously at followed 24h group,48h group compared with OA not added group.It seemed that there was a opposite relationship between PP2Ac and p-AKT.Furthermore,the results of immunohistochemical staining indicate there was no significant dependability(p＞0.05) in the expression of PP2Ac compared with sexuality,age and the degree of tumorous differentiation of lung cancer patients.The expression of PP2Ac was not observed in cytoplasm of squamous-carcinoma and minicell -undifferentiated carcinoma in lung cancer tissues,but PP2Ac-positive immunoreaction products can be seen in the cytoplasm.In lung adenocarcinoma,the expression of PP2Ac was negative.It is interesting to note that p-AKT was positive expression intensively in it.Conclusion:It is well known that PP2Ac plays an important role in antagonizing cancer,and it is an underlying gene against cancer. According to the consequences from the studies,we consider that PP2Ac involves in occurring of lung cancer possibly.Moreover,the expression level is associated with the different types of patho-lung cancer.The activation of p-AKT may be closely relevant to the occurrence and development of lung cancer.This studies provide experiment evidence for PP2Ac as a new target gene for diagnosis and treatment in lung cancer.