| Object TO investigate the expression of Rnd1, 2 in the brain tissue of intractable epilepsy and explore the pathogenic of intractable epilepsy(IE). Method Collecting brain tissue of 35 patients with intractable epilepsy,the expression levels of Rnd proteins1,2 are detected in IE group and normal control group by immunohistochemistry, immunofluorescence and Western-blot and compare to each other group. Result 1. Immunohistochemistry shows the average picture level of Rndl in the temporal lobe of the patient was127.62±6.23 significanfly higher than that of the controls (171.79±3.84, P<0. 01).The count of Positive staining cells in IE group is 30.20±3.42 higher than the control group(11.18±0.67).(P<0.01)Western-blot shows the ratio for optical density value of Rnd1 in epileptic patient tissue(0.52±0.034 ((?)±s)) was significantly higher (P<0.01)than that of control group. (0.23±0.015 ((?)±s)). Immunofluorescence shows the positive expression of Rnd1 distribute overlapping the distribution of NSE. 2.Immunohistochemistry shows the the average picture level of Rnd2 in the temporal lobe of the patient was 95.89±11.9 significantlyhigher than that of the controls(144.39±3.43, ((?)±s P<0. 01), the count of Positive staining cells in IE group is (23.47±3.9((?)±s))higher than the control group (11.18±0.67((?)±s)). (P＜0.01) Western-blot shows Rnd2 The ratio for optical density value of epileptic patient tissue (0.52±0.034 ((?)±s)) was significantly higher (P<0.01)than that of control group.(12.09±1.05((?)±s)). Immunofluorescence shows the positive expression of Rnd2distribute overlapping the distribution of NSE. Conclusion We found Rnd1 and Rnd2 up-regulate in the brain tissue of IE patients ,it suggested Rnd1,2 proteins may involve in the formation of intractable epilepsy pathogenic.
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