The Experiment Study On The Effects Of Resveratrol On The Epileptogenesis Of Pentylenetetrazole Induced Kindling Rats

Objective:To discuss the following things by establishing chemical kindling epilepsy model in rats by PTZ intraperitoneal injection.Firstly,to explore the mechanisms of epilepsy through observing the expression of nNOS in hippocampus of the epilepsia rats.Secondly,to discuss the effects of resveratrol on the expression of nNOS and the possible pharmacological effects of resveratrol.Thirdly,to explore the protective effect on neurons of resveratrol by observing the effects of resveratrol on behavior and EEG in Pentylenetetrazole induced chronic kindling rats.Medthods:Experiment 1:60 Wistar male rats were randomly divided into three groups:control group,model group and RV intervention group,20 rats in each group.To established acute animal model of epilepsy by intraperitoneal injection of PTZ.The expression of nNOS in hippocampus was investigated by immunohistochemistry and hematoxylin and eosin stain based on seizures in the schedule,5 rats per time point.Experiment 2:60 Wistar rats were divided into control group,model group and RV intervention group.Use chronic model of epilepsy induced by PTZ intra aperitoneal injection.Changes of behavior,EEG recording were observed.Results:Experiment 1:The levels of nNOS at various stages in PTZ kinding epilepsy group were obviously higher than those in normal group(P＜0.05);It reached the peak at reperfusion 24h;Compared with the normal group,AOD values at different times of ifenprodil group were decreased(P＜0.05).Experiment 2:In RV group,the epileptic discharge was decreased,the epileptic incubation period was prolonged,the duration was obviously reduced and the rate of serious seizure was lower(P＜0.05,Compared with group B).Conclusions:Experiment 1:①The expression of nNOS of hippocampal in PTZ kinding epilepsy rats was apparent increasing with different time points,which sug gested that nNOS may be involved in the formation of epilepsy;②resveratrol can significantly reduce the expression of nNOS positive cells,and reduce neuronal damage,which plays an very important role in anti-epileptic.Experiment 2:①The model of PTZ kindling epilepsy rats was easy to operate and can be regarded as an ideal epilepsy animal model;②Resveratrol can significantly inhibi ted epileptic discharge,extend the attack latency,shorten the attack time,reduce the amplitude,which can be used as a kind of neuronal protective agent.