The Preliminary Preparation On Test Strip Using Selenium Colloids Labeled McAb Against Microalbuminuria

At present, the fatality rate of diabetic is second to cardiovascular and cerebrovascular diseases and cancer. Diabetic has become the third largest disease, which is a threat to human health and life. Diabetic nephropathy (DN) is a systemic microvascular complications of diabetes as well as diabetic microangiopathy, which is one of the most dangerous complications. DN is a chronic progressive disease, so its clinical symptoms appear relatively late. Usually the urinary protein appears after 10 years. Once the urinary protein appears, the renal function will be more and more declining. Therefore it is important for the cure of DN that early detection and early treatment. Microalbuminuria (MAU) is that the albumin concentration in urine exceeded the range of healthy people, which can not be detected by conventional methods. MAU is recognized as an indicator of the early DN diagnosis, and its detection has important reference value and clinical significance.Currently, MAU can be detected by the methods of radioimmunoassay(RIA), enzyme-linked immunosorbent assay (ELISA) and immunoturbidimetric assay. RIA is short half-life and radioactive contamination; ELISA has lower detection limit (0.15 mg/L), but the detection rate is slow and not suitable for scene detection; Immuno turbidimetric is simple, rapid and repeatable, besides, more than 30 samples can be detected within 1 hour, but it needs high-quality protein analyzer. However, selenium colloid immunochromatography can overcome the drawbacks of the above three ways. At the same time, it has many advantages, such as fast detection, high sensitivity, specificity, good stability and easy operation.The detection result is reliable, economical and practical without any equipment. Appling routine immunization techniques, cell fusion technology and selective hybridoma clones, the experiment finally acquired four stable cell lines of monoclonal antibody anti-HAS: A4, A11, C8 and E4. We futherly studied the preparation, purification and identification of McAbs. Finally, the E4 and C8 were selected to the preparation of test paper. In the experiment, acid selenite was reduced to Colloidal selenium by ascorbic acid. The test strip was prepared by immunochromatography, selenium and standard double-antibody sandwich antibody. Using HAS as sample, the test strip achieved the certain results through the antigen-antibody interaction.