The Reforms And Biological Evaluation Of The Octyl-α-Cyanoacrylate Medical Adhesive

Objective:Octyl-α-Cyanoacrylate adhesive(OCA) is a kind of chemical adhesive used for soft tissue.Compared to other tissue adhesives,it is monomer and has attributes of rapid solidification,steady chemical character and good biocompatibility. In clinical application,it has already been used for the alternative of suture,fixation, hemostasia,blocking the leak and so on.The surgeons hope there is a kind of ideal medical adhesive,but the OCA adhesive in market now still can not satisfy the requirements.For example,the strength is not high enough,the facility after solidification is not very good,and neither the biocompatibility.Reformations for the OCA adhesive is studied in this dissertation,with the purpose of improving the adhesive strength,facility after solidification and biocompatibility.Methods:Three chemicals and two methods are chosen to reform the OCA adhesive,and agglutinate tests of the OCA before and after reformation have been carried.Then,certain groups were chosen as the evaluation of biocompatibility test group.The agglutinate tests include stretch shearing force test and adhesive strength test,and the biomedical evaluation includes tests for invitro cytotoxicity,hemolysis test,genetic-toxicity test,tests for irritation and delayed-type hypersensitivity,tests for systemic toxicity,embedded experiment and so on based on the GB/T 16886.Results:The PCL group in normal temperature is descended while the other groups are enhanced.And the intention of the rectified group is improved and has statistical difference compared to the OCA reformed before.The result of the biological evaluation indicates that,the vitro cytotoxicity,hemolysis,and the genetic-toxicity results of the OCA adhesive are up to grade.And the PCL group has low invitro cytotoxicity,while the POMA group has better hemolysis results.Conclusions:After the reformation,the adhesive strength and biocompatibility are promoted,which indicates that the reformation is effective.In order to guarantee the security of the OCA adhesive,the other biocompatibility tests are still needed.