Analysis Of Special Immune Effect In Mother-to-Child Living Renal Transplantation

Objective To compare one-center experience on kidney transplantation between mother-to-child and other relationships,and analyze incidence of microchimerism in renal transplantation recipients and its effect.We try to research the special immune effect in mother-to-child renal transplantation.Methods This retrospective study examined data of 130 recipients received allograft from living related donors from 2004 to 2008 in Zhongshan hospital. Recipients were followed up for half a year to four years.Demographic information about the study population was summarized using basic statistic method.We collected 43 samples of recipients after transplantation and used short tandem repeat(STR) to detect microchimerism.HLA-DRB1 locus were analyzed both by classical PCR-sequence-specific primers(SSP) and by PCR.Also,sex-determining region-y gene(SRY) gene allowed specific male donor discrimination in female recipients. According to the follow-up result,we analyzed its effect.Results Twenty-nine recipients took biopsy of the allograft,while 20 had acute rejection of different degrees.Thirteen recipients had clinical acute rejection but werenot proved by biopsy.Compared with outcomes,significant differences were demonstrated between mother-to-child and other relationships.Four samples were detected microchimerism in Sixteen mother-to-child recipients.While Four samples were detected microchimerism in twenty-seven other relationships recipients.In these recipients,there were 9 recipients with acute rejection.However,in our study,we failed to detect any effect of microchimerism in renal transplantation.Conclusions Compared with other relationships,Mother-to-child recipients had the higher acute rejection.And they had a higher incidence of microchimerism as well as a higher incidence of acute rejection.However we were not able to ascertain whether microchimerism actually associated with tolerance.However the special immune effect in mother-to child renal transplantation may influence its outcome.