The Extraction, Purification And Biologic Activity Of Ostreidae Polysaccharides

Oysters polysaccharide was extracted from oysters. In the article, we discussed the process of oysters polysaccharide extraction systematically and then analysed the purified polysaccharides's strcture , the biological activity in vivo and in vitro .We hope to provide polysaccharide reference for the further study of oysters medicinal value and oyster products.Crude polysacharides were isolated from Ostreidae by a method so called"water extraction followed by ethanol precipitation", Single factor test was used to analyze the influence of each factor, such as extraction times, solid-liquid ratio, and temperature. The optimal conditions for extraction and isolation of Ostreidae polysacharides (OPS) were studied by L933orthogonal experiment design.The results of orthogonal test on OPS showed that the optimal conditions for extraction of polysacharides from Ostreidae were as follows: temperature-90℃, solid-liquid ratio-1:80, extraction running times-3 hours.The polysacharide content of Ostreidae was determined by phenol-sulfuriic acid method. Finally, the content {percentage) of total sugar was determined to be 72.43%.After extraction, the free protein and other impurities in the crude extract were wiped out by Sevage method. After the process of dialyzing, the obtained polysaccharides were further purified by DEAE-52 column chromatography and Sephadex G-100 chromatography,OPS-II and OPS-II b were obtained. By using UV spectrum, it was found that there were no absorption peaks of protein and nucleic acids at 280 nm and 260nm. OPS-II b was tested by HPLC for purity and the result showed that OPS-IIb had a characteristic absorption peak, so the composition and structure of OPS-II b could be analyyzed.The physicochemistrical properties of OPS-IIb were preliminarily analyzed by HPLC, gas chromatography (GC), infrared spectroscopy (IR), thin layer chromaotography (TLC) and other Physical and chemical analysis methods, The results indicated that OPS-II b had typical absorption peaks,it is a component of glucose.The results of antioxidation test in vtro showed that OPS played a good role in the antioxidation process. OPS was obviously able to scavenge super oxide free radical in the concentration of 3.0mg/mL and the scavenging rate was 43.84%; while in the concentration of 4.0 mg/mL inhibition rate of hydroperoxide-induced hemolysis of red blood cells in mice and hydroxy free radical rate were 58.9% and 65.69%,respectively;the inhibition rate of liver lipid peroxidation reach 62.57%. Experiments in vitro showed that OPS could enhance macrophage phagocytosis, increase immune enzymatic activity of mice and increase their spleen indeces.Immune function test results in mice showed that the oyster 3mg/ml group can effectively increase the normal mouse spleen cells in Pakistan and carbon leaching dissection vitality, and 6mg/ml, 9mg/ml group had no significant effect.